全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用：99美元
投递稿件

查看量下载量

相关文章

更多...

瘤胃灌注大豆小肽对肉牛瘤胃发酵的影响

DOI: 10.3969/j.issn.1006-267x.2011.08.011

Keywords: 大豆小肽,肉牛,瘤胃发酵,瘤胃微生物

Full-Text   Cite this paper   Add to My Lib

Abstract:

本试验旨在探讨瘤胃灌注不同水平大豆小肽对肉牛瘤胃发酵指标和瘤胃微生物的影响。选用4头安装永久性瘤胃瘘管的鲁西黄牛阉牛，采用4×4拉丁方试验设计，分别瘤胃灌注0、100、200和300g/d的大豆小肽。采集瘤胃液，测定瘤胃发酵指标，实时定量PCR法测定瘤胃微生物相对含量。结果表明，灌注大豆小肽对瘤胃液pH平均值无显著影响（P>0.05），显著提高了瘤胃液氨态氮（NH3-N）与微生物蛋白（MCP）平均浓度（P<0.05）；灌注大豆小肽显著提高了瘤胃液总挥发性脂肪酸（TVFA）的浓度（P<0.05），与0g/d组相比，200g/d组和300g/d组丙酸百分含量显著增加（P<0.05），100g/d组和200g/d组乙酸百分含量显著降低（P<0.05），300g/d组丁酸含量显著降低（P<0.05）；灌注大豆小肽显著降低了乙酸/丙酸（P<0.05）；灌注小肽显著提高了瘤胃液中溶纤维丁酸弧菌含量（P<0.05）。可见，瘤胃灌注大豆小肽可促进MCP的合成、提高溶纤维丁酸弧菌的含量、改善瘤胃代谢。

 References

[1]  JONES D F, HOOVER W H, MILLER W T K. Effects of concentrations of peptides on microbial metabolism in continuous culture[J]. Journal of Animal Science, 1998, 76:611-616.
[2]  ALBERT L C, EDWARD P M. Modified reagents for determination of urea and ammonia[J]. Clinical Chemistry, 1962, 8(2):130-132.
[3]  秦为琳.应用气相色谱法测定挥发性脂肪酸的研究改进[J].南京农业大学学报,1982（5）：110-116.
[4]  ZINN R A, OWENS F N. A rapid procedure for purine measurement and its use for estimating net ruminal protein synthesis[J]. Canadian Journal of Animal Science, 1986, 66:157-166.
[5]  ZHOU J Z, BRUNS M A, JAMES M. DNA Recovery from soils of diverse composition[J]. Applied and environmental microbiology, 1996（2）:316-322.
[6]  NEWEY H, SMYTH D H. Intracellular hydrolysis of dipeptides during intestinal absorption[J]. Journal of Physiology, 1960, 152:367-374.
[7]  王玲,刘辉,李胜利,等.十二指肠灌注大豆小肽对奶山羊小肠肽吸收的影响[J].动物营养学报，2010,22(2):318-326.
[8]  PUCHALA R, PIERZYNOWSKI S G, WULIJI T, et al. Effects of small peptides or amino acids infused to a perfused area of the skin of Angora goats on mohair growth[J]. Journal of Animal Science, 2002, 80:1097-1104.
[9]  曹志军,李胜利,丁志明.日粮中添加小肽对奶牛产奶性能影响的研究[J].饲料工业,2004(4):35-37.
[10]  施用晖,乐国伟,左绍群,等.产蛋鸡日粮中添加酪蛋白肽对产蛋鸡性能及血浆肽和铁、锌含量的影响[J].四川农业大学学报,1996,14:46-50.
[11]  李丽立,陈宇光,谭支良,等.小肽对山羊氮平衡和营养物质消化率的影响[J].草业学报,2004,13(2):73-78.
[12]  郭春燕.羊血水解小肽的制备及其对绵羊瘤胃氮代谢的影响[D].硕士学位论文.乌鲁木齐:新疆农业大学,2006.
[13]  CRUZ S R, SAMIRAH M A, NEWBOLD C J, et al. Influence of peptides, amino acids and urea on microbial activity in the rumen of sheep receiving grass hay and on the growth of rumen bacteria in vitro[J]. Animal Feed Science and Technology, 1994, 49:151-161.
[14]  王梦芝,喻礼怀,王洪荣,等.不同分子形式氮源对瘤胃微生物发酵及蛋白合成的影响[J].中国畜牧杂志,2010,46(5):20-24.
[15]  马宁,单安山,马清泉,等.不同浓度肽对瘤胃发酵及微生物蛋白产量的影响[J].中国畜牧杂志,2009,45(15):29-32.
[16]  KHAFIPOUR E, LI S C, PLAIZIER J C, et al. Rumen microbiome composition determined using two nutritional models of subacute ruminal acidosis[J]. Applied and Environmental Microbiology, 2009, 75(22):7115-7124.
[17]  DENMAN S E, MCSWEENEY C S. Development of a real-time PCR assay for monitoring anaerobic fungal and cellulolytic bacterial populations within the rumen[J]. Federation of European Microbiological Societies Microbiology Ecology, 2006, 58:572-582.
[18]  李莉.体外法研究肽对瘤胃液pH、氨氮浓度、菌体蛋白氮浓度、中性洗涤纤维降解率及其产气量的影响[D].博士学位论文.呼和浩特:内蒙古农业大学,2001.
[19]  李海燕,徐凤霞,王中华.酪蛋白灌注水平对瘤胃蛋白质代谢的影响的研究[J].山东农业大学学报(自然科学版),2008,39(3):345-349.
[20]  PETERS J P, SHEN R Y, ROBINSON J A, et al. Disappearance and passage of propionic acid from the rumen of the beef steer[J]. Journal of Animal Science, 1990, 68:3373-3349.
[21]  BOWMAN J G, ASPLUND J M. Nitrogen utilization, ruminal fermentation and abomasal nitrogen flow in sheep fed Caucasian Bluestem hay supplemented with lucerne or urea[J]. Animal Feed Science and Technology, 1988, 20(1):33-44.
[22]  王加启,冯仰廉.日粮精粗比对瘤胃微生物合成效率的影响[J].畜牧兽医学报,1995,26(4):301-307.
[23]  ALLEN S A, MILLER E L. Determination of N requirement for microbial growth from the effect of urea supplementation of a low N diet on abomasal N flow and N recycling in wethers and lambs[J]. British Journal of Nutrition, 1976, 36:353.
[24]  IPHARRAGUERRE R, CLARK J H, FREEMAN D E. Varying protein and starch in the diet of dairy cows. I. Effects on ruminal fermentation and intestinal supply of nutrients[J]. Journal of Dairy Science, 2005, 88:2537-2555.
[25]  FU C J, FELTON E E, LEHMKUHLER J W, et al. Ruminal peptide concentration required to optimize microbial growth and efficiency[J]. Journal of Animal Science, 2001, 79:1305-1312.
[26]  李琍,丁角力.肽对体外混合培养瘤胃微生物发酵和生长影响的研究[J].畜牧兽医学报,2000(2):113-119.
[27]  GRISWOLD K E, MILLER T K, HOOVER W H, et al. Effect of form of nitrogen on growth of ruminal microbes in continuous culture[J]. Journal of Animal Science, 1996, 74:483-491.
[28]  夏楠,赵国琦.不同蛋白质组成的日粮对瘤胃发酵及微生物蛋白质合成的影响[J].中国畜牧兽医,2009,36(7):11-14.
[29]  RUSSELL J B, O''CONNOR J D, FOX D G, et al. Net carbohydrate and protein system for evaluating cattle diets: I. Ruminal fermentation[J]. Journal of Animal Science, 1992, 70(11):3551-3561.
[30]  邓茂昌,胡代泽,朗家文,等.生态条件和瘤胃丛密度与纤维分解的关系[J].资源开发与市场,1997,13(2):55-57.
[31]  王海荣.不同日粮精粗比及氮源对绵羊瘤胃纤维素降解菌群和纤维物质降解的影响[D].博士学位论文.呼和浩特:内蒙古农业大学,2007.
[32]  刘占英.绵羊瘤胃主要纤维素降解细菌的分离鉴定及不同氮源对其纤维降解能力的影响[D].博士学位论文.呼和浩特:内蒙古农业大学,2008.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133