全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

饲料中添加乳酸杆菌对幼建鲤生长性能和消化吸收功能的影响

DOI: 10.3969/j.issn.1006-267x.2011.08.018

Keywords: 幼建鲤,乳酸杆菌,生长性能,消化吸收

Full-Text   Cite this paper   Add to My Lib

Abstract:

本试验旨在研究饲料中添加乳酸杆菌对幼建鲤生长性能和消化吸收功能的影响。选择平均体重为(22.35±0.06)g的健康幼建鲤720尾,随机分成6组,每组设3个重复,每个重复40尾,分别饲喂乳酸杆菌含量为0(对照组)、0.45×107、0.91×107、1.31×107、2.14×107和2.40×107cfu/kg的试验饲料80d。结果表明,各乳酸杆菌组的末重、增重率和摄食量均显著或极显著高于对照组(P<0.05或P<0.01)。当乳酸杆菌含量>0.45×107cfu/kg时,幼建鲤蛋白质沉积率和脂肪沉积率均极显著提高(P<0.01);当乳酸杆菌含量>0.91×107cfu/kg时,幼建鲤灰分沉积率极显著高于对照组(P<0.01)。饲料中添加乳酸杆菌可以显著或极显著提高幼建鲤肝胰脏和肠重、肝胰脏和肠蛋白质含量以及前、中、后肠皱襞高度(P<0.05或P<0.01)。当乳酸杆菌含量>0.45×107cfu/kg时,肠道糜蛋白酶、脂肪酶和淀粉酶活性极显著升高(P<0.01);当乳酸杆菌含量>0.91×107cfu/kg时,肠道胰蛋白酶活性显著升高(P<0.05)。对照组前、中、后肠碱性磷酸酶、Na+,K+-ATP酶、γ-谷氨酰转肽酶以及全肠肌酸激酶活性显著或极显著低于各乳酸杆菌组(P<0.05或P<0.01)。由此得出,饲料中添加乳酸杆菌可以促进幼建鲤的生长,同时促进其消化器官的生长发育,提高幼建鲤的消化和吸收能力。以幼建鲤增重率为标识,根据折线法确定的乳酸杆菌最适添加量为0.99×107cfu/kg。

References

[1]  DHANARAJ M, HANIFFA M A, SINGH S V A, et al. Effect of probiotics on growth performance of koi carp (Cyprinus carpio)[J]. Journal of Applied Aquaculture, 2010, 22(3):202-209.
[2]  RAMAKRISHNAN C M, HANIFFA M A, MANOHAR M, et al. Effects of probiotics and spirulina on survival and growth of juvenile common carp (Cyprinus carpio)[J]. The Israeli Journal of Aquaculture, 2008, 60(2):128-133.
[3]  EL-HAROUN E R, GODA A M, CHOWDURY M A K. Effect of dietary probiotic Biogen supplementation as a growth promoter on growth performance and feed utilization of Nile tilapia Oreochromis niloticus (L)[J]. Aquaculture Research, 2006, 37:1473-1480.
[4]  GUARNER F, SCHAAFSMA G F. Probiotics[J]. International Journal of Food Microbiology, 1993, 39:237-238.
[5]  CARNEVALI O, SULPIZIO R, GIOACCHINI G, et al. Growth improvement by probiotic in European sea bass juveniles (Dicentrarchus labrax, L.), with particular attention to IGF-1, myostatin and cortisol gene expression[J]. Aquaculture, 2006, 258:430-438.
[6]  SUZER C, COBAN D, KAMACI H O, et al. Lactobacillus spp. bacteria as probiotics in gilthead sea bream (Sparus aurata, L.) larvae: effects on growth performance and digestive enzyme activities[J]. Aquaculture, 2008: 116-124.
[7]  ALY S M, AHMED Y, GHAREEB A, et al. Studies on Bacillus subtilis and Lactobacillus acidophilus, as potential probiotics, on the immune response and resistance of Tilapia nilotica (Oreochromis niloticus) to challenge infections[J]. Fish & Shellfish Immunology, 2008, 25:128-136.
[8]  林浩然.鱼类生理学[M].广州:广东高等教育出版社,1998:37-43.
[9]  华雪铭,周洪琪,张宇峰,等.饲料中添加壳聚糖和益生菌对暗纹东方鲀幼鱼生长及部分消化酶活性的影响[J].水生动物学报,2005,29(3):299-305.
[10]  OLLI J J, KROGDAHL A. Nutritive value of four soybean products as protein sources in diets for rainbow trout (Oncorhynchus mykiss, Walbaum) reared in fresh water[J]. Animal Science, 1994, 44(3):185-192.
[11]  CUVIER-PRES A, KESTEMONT P. Development of some digestive enzymes in Eurasian perch larvae Perca fluviatilis[J]. Fish Physiology and Biochemistry, 2001, 24(4):279-285.
[12]  SUZER C, AKTULUN S, COBAN D, et al. Digestive enzyme activities in larvae of sharpsnout seabream (Diplodus puntazzo)[J]. Comparative Biochemistry and Physiology—Part A: Molecular & Integrative Physiology, 2007, 148(2):470-477.
[13]  VEILLETTE P A, YOUNG G. Temporal changes in intestinal Na+,K+-ATPase activity and in vitro responsiveness to cortisol in juvenile chinook salmon[J]. Comparative Biochemistry and Physiology—Part A: Molecular & Integrative Physiology, 2004, 138(3):297-303.
[14]  孙建广,张石蕊,谯仕彦,等.发酵乳酸杆菌对生长肥育猪生长性能和肉品质的影响[J].动物营养学报,2010,22(1):132-138.
[15]  倪学勤,曹希亮,曾东,等.猪源约氏乳酸杆菌JJB3和枯草芽孢杆菌JS01对仔猪肠道分泌型免疫球蛋白A和菌群的影响[J].动物营养学报,2008,20(3):275-280.
[16]  PANIGRAHIA A, KIRON V, KOBAYASHI T, et al. The viability of probiotic bacteria as a factor influencing the immune response in rainbow trout Oncorhynchus mykiss[J]. Aquaculture, 2005, 243:241-254.
[17]  张军民.谷氨酰胺对早期断奶仔猪肠道的保护作用及其机理研究[D].博士学位论文.北京:中国科学院,2005.
[18]  王坤.实用酶学诊断学[M].上海:医科大学出版社,2000.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133