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饲粮结构对山羊消化系统尿素转运蛋白-B表达的影响

DOI: 10.3969/j.issn.1006-267x.2011.05.024

Keywords: 饲粮结构,山羊,消化系统,UT-B

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Abstract:

本试验旨在研究饲粮结构对山羊消化系统尿素转运蛋白-B(UT-B)表达的影响。选用18只装有永久性瘤胃瘘管的内蒙古白绒山羊半同胞羯羊,随机分为6组(每组3个重复,每个重复1只羊),45d后每组随机屠宰1只山羊,采用蛋白质印迹分析所采集的样品中UT-B的表达情况,并对34kuUT-B进行相对定量分析。结果表明:所有样品表达的UT-B处于30~55ku且均含有34ku的UT-B;饲粮高蛋白质水平可提高前胃(瘤胃、网胃和瓣胃)、回肠、盲肠盲囊和肾脏中34kuUT-B的表达;玉米膨化加工减少了34kuUT-B在回肠和肝脏的表达;各组织中34kuUT-B分布由高到低依次为肝脏、皱胃、网胃、瘤胃背囊、瘤胃腹囊、腮腺、瓣胃、十二直肠、盲肠、盲肠盲囊、回肠和肾脏。由此可知,通过改变饲粮结构可以调控山羊消化系统各组织中UT-B的表达,为进一步研究尿素循环和机体氮代谢提供依据。

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