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饲料中添加核苷酸混合物对凡纳滨对虾幼虾非特异性免疫和抗氧化指标的影响

DOI: 10.3969/j.issn.1006-267x.2011.05.017

Keywords: 凡纳滨对虾,核苷酸混合物,非特异性免疫指标,抗氧化指标

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Abstract:

本试验旨在研究外源核苷酸混合物对凡纳滨对虾(Litopenaeusvannamei)幼虾非特异性免疫和抗氧化指标的影响。选取960尾初始体重为(0.43±0.01)g的凡纳滨对虾,随机分为8组(每组设3个重复,每个重复40尾虾),分别投喂在基础饲料中添加0(对照)、0.1、0.2、0.4、0.6、0.8、1.0和1.2g/kg核苷酸混合物(5′-腺苷酸∶5′-胞苷酸∶5′-尿苷酸二钠∶5′-肌苷酸二钠∶5′-鸟苷酸二钠=1∶1∶1∶1∶1,质量比)的试验饲料,养殖期为5周。结果表明,饲料中添加0.4~1.2g/kg核苷酸混合物可显著或极显著提高凡纳滨对虾幼虾血细胞总数(THC)(P<0.05或P<0.01)。与对照组相比,0.6和0.8g/kg组血清超氧化物歧化酶(SOD)活性显著升高(P<0.05)。0.4g/kg组血清总抗氧化能力(T-AOC)与对照组相比提高了51.0%(P>0.05),并显著高于1.2g/kg组(P<0.05)。各组肝胰腺SOD活性和T-AOC随饲料中核苷酸混合物添加量的增加先升高后降低,均在0.4g/kg组达到最高值。各组血清和肝胰腺过氧化物酶(POD)和碱性磷酸酶(AKP)活性均无显著差异(P>0.05)。饲料中添加0.2~1.2g/kg核苷酸混合物可显著降低对虾肝胰腺丙二醛(MDA)含量(P<0.05),而各组血清MDA含量差异不显著(P>0.05)。0.2、0.4和0.6g/kg组肌肉抗超氧阴离子自由基(O-2·)活性显著高于1.0和1.2g/kg组(P<0.05),其他各组间差异不显著(P>0.05)。结果提示,饲料中添加一定量的核苷酸混合物能提高凡纳滨对虾幼虾的非特异性免疫和抗氧化能力,建议添加量为0.4~0.6g/kg。

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