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长期投喂黄芪多糖对黄颡鱼免疫细胞活性的影响

DOI: 10.3969/j.issn.1006-267x.2011.05.026

Keywords: 黄颡鱼,黄芪多糖,免疫细胞活性,呼吸爆发,白细胞增殖

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Abstract:

本试验旨在研究黄芪多糖(Astragaluspolysaccharides，APS)对黄颡鱼(Pelteobagrusfulvidraco)免疫细胞活性的影响。540尾鱼被随机分成6组，每组3个重复，每个重复30尾鱼，分别投喂添加0、300、600、900、1200、1500mg/kgAPS的饲料，饲养期为8周。采用密度梯度离心技术分离纯化黄颡鱼头肾巨噬细胞与外周血液白细胞；分别采用硝基蓝四氮唑(NBT)法、格里斯氏（Griesse）试剂法和噻唑蓝（MTT）比色法测定头肾巨噬细胞氧、氮呼吸爆发活性和外周血白细胞的增殖能力。结果表明：各APS组的黄颡鱼头肾巨噬细胞氧、氮呼吸爆发活性均高于空白对照，但仅1200mg/kg组鱼巨噬细胞氧呼吸爆发活性显著提高(P<0.05)；1200和1500mg/kgAPS组氮呼吸爆发活性极显著提高(P<0.01)；投喂黄芪多糖能显著提高外周血白细胞的增殖能力(P<0.05)，且1200和1500mg/kg组增殖效果尤为显著(P<0.01)。结果提示，APS可以有效地提高黄颡鱼免疫细胞的活性，最佳剂量为1200mg/kg。

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