全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

维生素C对建鲤肠上皮细胞氧化损伤保护作用的研究

DOI: 10.3969/j.issn.1006-267x.2012.08.014

Keywords: 建鲤,维生素C,肠上皮细胞,抗氧化能力

Full-Text   Cite this paper   Add to My Lib

Abstract:

建鲤肠上皮细胞(IEC)在24孔培养板中原代培养72h并更换无血清DMEM培养液继续培养12h后,随机分为7组,每组4个重复。除其中1组作为空白对照外,另外6组均加入100μmol/L的过氧化氢(H2O2)以建立IEC氧化应激模型。相同条件下继续培养16h后,将培养液分别更换为含0(空白对照组)、0、4、10、16、22和28mg/L维生素C的无血清DMEM培养液,相同条件下继续培养72h后取样测定指标,以考察不同维生素C浓度对H2O2诱导的建鲤IEC氧化损伤的保护作用。结果显示:1)100μmol/LH2O2处理导致IEC存活数量减少,仅形成一些小细胞集落,并显著降低IEC中超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽硫转移酶和谷胱甘肽还原酶活性以及谷胱甘肽和蛋白质含量(P<0.05),提高IEC中丙二醛及蛋白质羰基含量以及培养液中乳酸脱氢酶活性(P<0.05),使建鲤IEC产生了氧化应激;2)维生素C显著提高IEC的抗羟自由基能力和抗超氧阴离子自由基能力(P<0.05),缓解由H2O2导致的建鲤IEC氧化损伤。综上可见,维生素C可提高建鲤IEC抗氧化能力,有效保护IEC免受氧化损伤。

References

[1]  GLISZCZY N ' SKA-S ' WIGLO A.Antioxidant activity of water soluble vitamins in the TEAC (trolox equivalent antioxidant capacity) and the FRAP (ferric reducing antioxidant power) assays[J].Food Chemistry,2006,96:131-136.
[2]  王秋林,王浩毅,王树人,等.氧化应激状态的评价[J].中国病理生理杂志,2005,21:2069-2074.
[3]  MITRA G,MUKHOPADHYAY P K,AYYAPPN S.Modulation of digestive enzyme activities during ontogeny of Labeo rohita larvae fed ascorbic acid enriched zooplankton[J].Comparative Biochemistry Physiology,2008,149A:341-350.
[4]  KOHEN W,ROJAS-GARCIA C R,FIN R N,et al.Stimulatory effect of ingested protein and/or free amino acids on the secretion of the gastro-endocrine hormone cholecystokininand on tryptic activity,in early feeding herring larvae,Clupea harengus[J].Marine Biology,2002,140:1241-1247.
[5]  DABROWSKI K,GUDERLEY H.Intermediary metabolism[M]//HALVER J E,HARDY R.Fish nutrition.New York:Academic Press,2002:309-365.
[6]  郑婷.维生素E对建鲤肠上皮细胞生长发育及抗氧化能力的影响 .硕士学位论文.雅安:四川农业大学,2007.
[7]  LIU Y,CHI L,FENG L,et al.Effects of graded levels of dietary vitamin C on the growth,digestive capacity and intestinal microflora of juvenile Jian carp (Cyprinus carpio var.Jian)[J].Aquaculture Research,2011,42:534-548.
[8]  姜俊.谷氨酰胺对建鲤肠上皮细胞增殖和分化的影响 .硕士学位论文.雅安:四川农业大学,2005.
[9]  AW T Y.Cellular redox:a modulator of intestinal epithelial cell proliferation[J].News in Physiological Sciences,2003,18:201-204.
[10]  冯琳.大豆凝集素对鲤鱼肠上皮细胞增殖和分化的影响 .硕士学位论文.雅安:四川农业大学,2006.
[11]  BASIVIREDDY J,JACOB M,BALASUBRAMANIAN K A,et al.Oral glutamine attenuates indomethacin induced small intestinal damage[J].Clinical Science,2004,107:281-289.
[12]  陈瑾.谷氨酰胺对鲤鱼肠上皮细胞抗氧化能力的影响 .硕士学位论文.雅安:四川农业大学,2008.
[13]  BALTACIO G ˇ LU E,AKALIN F A,ALVER A,et al.Protein carbonyl levels in serum and gingival in patients with chronic periodontitis[J].Archives of Oral Biology,2008,53:716-722.
[14]  BAI J,CEDERBAUM A I.Mitochondrial catalase and oxidative injury[J].Biological Signals and Receptors,2001,10:189-199.
[15]  WINSTON G W,DI-GIULIO R T.Prooxidant and antioxidant mechanisms in aquatic organisms[J].Aquatic Toxicology,1991,19:137-161.
[16]  ELIA A C,ANASTASI V,DRR A J M.Hepatic antioxidant enzymes and total glutathione of Cyprinus carpio exposed to three disinfectants, chlorine dioxide, sodium hypochlorite and peracetic acid for superficial water potabilization[J].Chemosphere,2006,64:1633-1641.
[17]  杨雨晗,王玉明,郑人源,等.维生素C对神经元细胞生长的时间剂量效应[J].营养学报,2009(5):460-464.
[18]  HUANG J, MAY J M.Ascorbic acid spares alpha-tocopherol and prevents lipid peroxidation in cultured H4IIE liver cells[J].Molecular and Cellular Biochemistry,2003,247:171-176.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133