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不同硒源及硒水平对大鼠生长性能、血清抗氧化能力和组织硒沉积的影响

DOI: 10.3969/j.issn.1006-267x.2012.07.016

Keywords: 亚硒酸钠,酵母硒,谷胱甘肽过氧化物酶,组织硒含量,生物学效价

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Abstract:

本试验旨在考察不同硒源及硒水平对大鼠生长性能、血清抗氧化能力及组织硒沉积的影响,并以亚硒酸钠和商品酵母硒(酵母硒B)为参比,对本课题组用糖蜜和尿素为发酵底物研制的酵母硒(酵母硒A)生物学效价进行评定。将硒耗竭2周后的70只8周龄左右Wistar雌性大鼠称重,随机分到10个处理,每个处理7个重复,每个重复1只大鼠,分别饲喂不同处理的饲粮21d。处理1不添加任何形式的硒源,处理2~9在基础饲粮中分别以亚硒酸钠或酵母硒A的形式添加0.1、0.2、0.3和0.4mg/kg硒,处理10以酵母硒B形式添加0.3mg/kg硒。结果表明:1)不同硒源及硒水平对大鼠生长性能、血清谷胱甘肽过氧化物酶(GSH-Px)和超氧化物歧化酶活性影响不显著(P>0.05);2)组织硒含量随饲粮硒水平的升高极显著增加(P<0.01),添加酵母硒A组大鼠组织硒含量显著高于亚硒酸钠组(P<0.05);3)以亚硒酸钠为参比,血清GSH-Px活性及肝脏、肾脏和肌肉硒含量作为判定指标,酵母硒A的相对生物学效价分别为95.9%、127.5%、114.5%和101.2%;4)添加酵母硒A和酵母硒B对大鼠生长性能、血清抗氧化能力的影响不显著(P>0.05),但添加酵母硒A组大鼠肾脏硒含量极显著高于酵母硒B组(P<0.01),而酵母硒B在肌肉中有更多的硒沉积(P<0.01)。结果提示:1)硒源对大鼠组织硒沉积有较大影响,酵母硒相对于亚硒酸钠具有更高的生物学效价,但不同酵母硒之间存在一定的差异;2)饲粮硒水平对大鼠血清抗氧化能力影响较小,以血清GSH-Px活性为衡量标准,0.1mg/kg硒的酵母硒和亚硒酸钠都能满足大鼠的需要,但更高水平的酵母硒可以使大鼠组织中有更多硒的储备。

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