全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

γ-氨基丁酸对断喙雏鸡血清细胞因子含量及脾脏中Bcl-2和Fas基因mRNA表达量的影响

DOI: 10.3969/j.issn.1006-267x.2012.07.021

Keywords: γ-氨基丁酸,断喙应激,细胞因子,细胞凋亡,雏鸡

Full-Text   Cite this paper   Add to My Lib

Abstract:

本试验旨在研究γ-氨基丁酸(GABA)对断喙应激条件下雏鸡生长性能、血清细胞因子含量及脾脏中细胞凋亡相关基因B细胞淋巴瘤/白血病2(B-celllympoma/leukemia-2,Bcl-2)和Fas基因mRNA表达量的影响。选取1日龄体重相近的健康固始鸡公雏360只,随机分为5个组,每组6个重复,每个重复12只鸡。其中A、B、C组均为断喙组,饮水中分别添加40、60和80mg/kg的GABA;D组不断喙,不添加GABA;E组断喙,不添加GABA。结果表明:1)断喙应激导致雏鸡采食量减少、体重下降,添加GABA可以提高采食量和体重;2)断喙应激引起了血清白细胞介素1β(IL-1β)、白细胞介素6(IL-6)和肿瘤坏死因子α(TNF-α)含量的升高,第1、5天E组血清IL-1β含量显著高于D组(P<0.05),添加80mg/kgGABA显著降低了血清IL-1β含量(P<0.05);与D组相比,断喙后第1、7天E组血清TNF-α和IL-6含量均显著升高(P<0.05),添加80mg/kgGABA后显著降低(P<0.05);3)E组第1、3、7天脾脏中促凋亡基因FasmRNA表达量显著高于D组,添加GABA后下降,且随着GABA剂量加大而进一步降低(P<0.05),添加80mg/kgGABA可轻度上调抗凋亡基因Bcl-2mRNA表达量(P>0.05)。由此可知,GABA在改善断喙应激雏鸡生长性能方面具有良好效果,同时添加GABA抑制了断喙应激后雏鸡血清细胞因子含量以及脾脏中促凋亡基因Fas的mRNA表达量的升高,且添加80mg/kg效果较好。

References

[1]  HESTER P Y,SHEA-MOORE M.Beak trimming egg-laying strains of chickens[J].World’s Poultry Science,2003,59:458-474.
[2]  GENTLE M J,WADDINGTON D,HUNTER L H,et al.Behavioural evidence for persistent pain following partial beak amputation in chickens[J].Applied Animal Behaviour Science,1990,27:149-157.
[3]  GENTLE M J,HUNTER L N,WADDINGTON D.The onset of pain related behaviours following partial beak amputation in the chicken[J].Neuroscience Letters,1991,128:113-116.
[4]  GONG C,HOFF J T,KEEP R F.Acute inflammatory reaction following experimental intracerebral hemorrhage in rat[J].Brain Research,2000,871(1):57-65.
[5]  吴家幂,马领松,周向阳,等.补体在脑出血后脑组织损伤机制中的作用[J].中华神经医学杂志,2006,5(2):152-154.
[6]  孙桂荣,李燕,康相涛,等.断喙应激对雏鸡胸腺细胞凋亡及相关凋亡蛋白表达的影响[J].畜牧兽医学报,2011,42(7):1000-1006.
[7]  GENTLE M J.Cutaneous sensory afferents recorded from the nervus intramandibularis of Gallus gallus var domesticus[J].Comparative Physiology A,1989,164:763-774.
[8]  XU X G,YANG Z F,HUANG S H,et al.Promotive effects of GABA on acid secretion from isolated mouse stomach in vitro[J].Acta Zoologica Sinica,2001,47:170-175.
[9]  ZAKUSOV W,OATROVSKAYA R U,BULAYEV V M.GABA-opiates interactions in the activity of analgesics[J].Archives Internationales de Pharmacodynamie et de Therapie,1983,265:61-75.
[10]  CHERUBINI E,GAIARSA J L,BEN-ARI Y.GABA:an excitatory transmitter in early postnatal life[J].Trends in Neurosciences,1991,12:515-519.
[11]  FAN Z Y,DENG J P,LIU G H,et al.Effects of γ-aminobutyric acid on the performance and internal hormone levels in growing pigs[J].Chinese Journal of Animal Nutrition,2007,19(4):350-356.
[12]  PIGUERAS L,VICENTE M.Peripheral GABA-β agonists stimulate gastric acid secretion in mice[J].British Journal of Pharmacology,2004,142:1038-1048.
[13]  KATO S,ARAKI H,KAWAUCHI S.Body temperature dependence in baclofen-induced gastric acid secretion in rats relation to capsaicin sensitive affront neurons[J].Life Sciences,2001,68:1951-1963.
[14]  SHUYE P U,JAIN M R,HORVATH T L.Interactions between neuropeptide Y and γ-ammobutyric acid in stimulation of feeding:a morphological and pharmacological analysls[J].Endocrinology,1999,140:933-940.
[15]  KHANSARI D N,MURGO A J,FAITH R E.Effects of stress on the immune system[J].Immunol Today,1990,11:170-175.
[16]  CUNHA F Q,FERREIRA S H.Peripheral hyperalgesic cytokines[J].Advances in Experimental Medicine and Biology,2003,521:22-39.
[17]  ARRUDA J,SWEITZER L S,RUTKOWSKI M D,et al.Intrathecal anti-IL-6 antibody and IgG attenuates peripheral nerve injury-induced mechanical allodynia in the rat:possible immune modulation in neuropathic pain[J].Brain Research,2000,879:216-225.
[18]  FRISEN J,RISLING M,FRIED K.Distribution and axonal relations of macrophages in a neuroma[J].Neuroscience,1993,55:1003-1013.
[19]  SPANGELO B L,HORRELL S,GOODWIN A L,et al.Somatostatin and gamma-aminobutyric acid inhibit interleukin-1β-stimulated release of interleukin-6 from rat c6 glioma cells[J].Neuroimmunomodulation,2004,11:332-340.
[20]  JOSEPH D,ROACH J D,AGUINALDO G T,et al.γ-aminobutyric acid inhibits synergistic interleukin-6 releases but not transcriptional activation in astrocytoma cells[J].Neuroimmunomodulation,2008,15:117-124.
[21]  ITOH N,NAGATA S.A novel protein domain required for apoptosis[J].Biological Chemistry,1993,268:10932-10939.
[22]  WILLIAMS G T,SMITH C A.Molecular of apoptosis: genetic controls on cell death[J].Cell,1993,74:777-779.
[23]  胡书超,张建祥.脑出血后的神经细胞凋亡机制及治疗策略[J].国外医学脑血管疾病分册,2004,12(7):524-526.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133