全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

精氨酸水平对奶牛乳腺上皮细胞体外生长及κ-酪蛋白基因表达的影响

DOI: 10.3969/j.issn.1006-267x.2012.05.009

Keywords: 乳腺上皮细胞,精氨酸,细胞增殖,κ-酪蛋白基因,基因表达

Full-Text   Cite this paper   Add to My Lib

Abstract:

本试验旨在研究培养基中添加不同浓度的精氨酸(Arg)对乳腺上皮细胞体外增殖及κ-酪蛋白(CSN3)基因表达的影响。选用中国荷斯坦奶牛乳腺上皮细胞进行体外培养,以无Arg的培养基(0.00mg/L)为对照(0组),试验培养基分别添加69.50(0.25组)、139.00(0.50组)、278.00(1.00组)、556.00(2.00组)、1112.00(4.00组)和2224.00mg/L(8.00组)的精氨酸。结果表明:Arg能促进乳腺上皮细胞的增殖,24h时,0.25~4.00组与0组相比均差异显著(P<0.05),8.00组与0组相比差异不显著(P>0.05);48和72h时,各试验组与0组相比均差异显著(P<0.05)。Arg能促进CSN3基因的表达,各试验组与0组相比均差异显著(P<0.05),当Arg浓度为556.00mg/L时,CSN3基因表达量最高。结果提示,Arg对乳腺上皮细胞增殖及CSN3基因表达均具有明显的促进作用,且在Arg浓度为69.50~1112.00mg/L时促细胞增殖效果较佳,在Arg浓度为556.00mg/L时促CSN3基因表达作用最强。

References

[1]  WU G,OTT T L,KNABE D A.Amino acid composition of the fetal pig[J].The Journal of Nutrition,1999,129(5):1031-1038.
[2]  WU G,POND W G,FLYNN S P.Maternal dietary protein deficiency decreases nitric oxide synthase and ornithine decarboxy-lase activities in placenta and endometrium of pigs during early gestation[J].The Journal of Nutrition,1998,128(12):2395-2402.
[3]  MATEO R D,WU G,BAZER F W.Dietary L-arginine supplementation enhances the reproductive performance of gilts[J].The Journal of Nutrition,2007,137(3):652-656.
[4]  O''QUINN P R,KNABE D A,WU G.Arginine catabolism in lactating porcine mammary tissue[J].Journal of Animal Science,2002,80(2):467-474.
[5]  YU L B,DONG X S,SUN W Z.Effect of a nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester on invasion of human colorectal cancer cell line SL-174T[J].World Journal of Gastroenterology,2005,11(40):6385-6388.
[6]  WU G,MEININGER C J.Arginine nutrition and cardiovascular function[J].The Journal of Nutrition,2000,130(11):2626-2629.
[7]  BEQUETTE B J,KYLE C E,MACRAE J C,et al.Vascular sources of phenylalanine,tyrosine,lysine,and methinoine for casein synthesis in lactating goats[J].Journal of Dairy Science,1999,82(2):362-377.
[8]  MABJEESH S J,KYLE C E,MACRAE J C,et al.Vascular sources of amino acid for milk protein synthesis in goats at two stages of lactation[J].Journal of Dairy Science,2002,85(4):919-929.
[9]  但堂胜.各种动物的理想蛋白及供给[J].国外畜牧科技,1997,24(2):2-6.
[10]  董矜,田亚平,房征宇.L-Arg对Hela细胞增殖的调控作用研究[J].军医进修学院学报,2004,25(2):100-102.
[11]  罗海吉,周爱军,吉鸿雁.高温下精氨酸对一氧化氮和细胞增殖的影响[J].中国公共卫生,2005,21(3):304-305.
[12]  BROCKMAN J L,SCHROEDER M D,SCHULER L A.PRL activates the cyclinD1 promoter via the Jak2/Stat pathway[J].Molecular Endocrinology,2002,16(14):774-784.
[13]  BUSER A C,GASS-HANDEL E K,WYSZOMIERSKI S L,et al.Progesterone receptor repression of prolactin/signal transducer and activator of transcription 5-mediated transcription of the beta-casein gene in mammary epithelial cells[J].Molecular Endocrinology,2007,21(1):106-125.
[14]  何晋,谢秀梅,方叶青,等.L-Arg抑制非对称性二甲基Arg对内皮祖细胞的增殖作用[J].中国动脉硬化杂志,2007,15(10):751-754.
[15]  张剑,何生,李茂德.L-Arg在人肝癌细胞生长增殖中的作用[J].贵阳医学院学报,2003,28(1):5-8.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133