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鲈鱼视黄酸受体α和视黄酸受体γcDNA克隆和基因表达分析

DOI: 10.3969/j.issn.1006-267x.2012.04.016

Keywords: 维生素A,视黄酸受体,克隆,表达,鲈鱼

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Abstract:

本文旨在研究鲈鱼视黄酸受体α(RARα)和视黄酸受体γ(RARγ)的结构及其基因的组织表达特点。采用反转录PCR和cDNA末端快速克隆(RACE)技术,从鲈鱼肝脏中克隆得到RARα和RARγ全长cDNA序列。检测鲈鱼肝脏、肌肉、心脏、眼、肠、肾脏、脂肪、脾脏、鳃和大脑10个组织中RARα和RARγ基因的表达情况。结果表明:1)鲈鱼RARαcDNA全长2094bp,5'端和3'端的非翻译区分别为124和608bp,开放阅读框为1362bp,推测编码453个氨基酸,分子质量为50.64ku,理论等电点为8.20。2)RARγcDNA全长1671bp,其中包括36bp的5'端非翻译区、129bp的3'端的非翻译区和1506bp的开放阅读框,共编码501个氨基酸,分子质量为56.20ku,理论等电点为4.96。3)鲈鱼RARα和RARγ都具有典型的核受体结构,两者氨基酸序列同源性高达63.1%。鲈鱼的RARα和RARγ与红鳍东方鲀有较高的同源性,分别为96.9%和95.2%。4)RARα和RARγ基因在所有检测组织中均有表达,其中RARα基因在心脏和大脑中表达较少,在眼、鳃和肠中表达较高,而RARγ基因仅在鳃和脾脏中有较高表达。总之,本试验成功克隆了鲈鱼RARα和RARγ的全长cDNA;鲈鱼RARα和RARγ有着典型的核受体结构,在各组织中广泛分布。

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