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不同外源寡糖对崂山奶山羊瘤胃微生物区系的影响

DOI: 10.3969/j.issn.1006-267x.2012.02.023

Keywords: 奶山羊,寡糖,微生物区系,瘤胃液

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Abstract:

本试验旨在研究不同外源寡糖对奶山羊瘤胃微生物区系的影响。试验选用6只平均体重为(32.80±2.45)kg、装有永久性瘤胃瘘管的崂山奶山羊,采用分期分组试验设计。各组羊饲喂基础饲粮(对照组)以及分别在基础饲粮中添加1%甘露寡糖(MOS组)、半乳甘露寡糖(GMOS组)、寡木糖(XOS组)、低聚异麦芽糖(IMO组)、果寡糖(FOS组)的试验饲粮。共进行4期试验,每期23d,其中预试期14d,正试期9d。于晨饲前(0h),晨饲后2、4、6、8h以及晚饲前(晨饲后10h)采集瘤胃液,测定瘤胃液中细菌总数以及纤维素分解菌、原虫和真菌数量。结果表明:与对照组相比,试验组瘤胃液细菌总数趋于增加,仅晨饲前MOS组显著提高(P<0.05);试验组瘤胃液纤维素分解菌数量有所提高,其中MOS组(0h)、GMOS组(0、2、4h)、FOS组(0、2、4h)显著高于对照组(P<0.05);试验组瘤胃液原虫数量有所提高,其中MOS组(2和4h)、XOS组(4h)显著高于对照组(P<0.05);试验组瘤胃液真菌数量有所提高,其中GMOS组和FOS组(0h)极显著高于对照组(P<0.01),MOS组(2h)、IMO组(2h)、FOS组(2h)、MOS组(4h)、FOS组(6h)显著高于对照组(P<0.05)。细菌总数、纤维素分解菌和原虫数量呈先下降后上升趋势,真菌数量呈先上升后下降趋势。本试验条件下,添加不同外源寡糖对瘤胃微生物影响有所差异,其中甘露寡糖、半乳甘露寡糖和果寡糖对瘤胃液纤维素分解菌、原虫和真菌的增殖作用较明显。

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