全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

鱼粉蛋白与发酵酶解豆粕蛋白不同配比对日本沼虾生长及免疫性能的影响

DOI: 10.3969/j.issn.1006-267x.2015.01.019

Keywords: 日本沼虾,发酵酶解豆粕,鱼粉,生长性能,免疫力

Full-Text   Cite this paper   Add to My Lib

Abstract:

本试验旨在评价补充微囊氨基酸条件下鱼粉蛋白和发酵酶解豆粕蛋白不同配比对日本沼虾生长和免疫性能的影响。试验配制5组等氮等能饲料,以发酵酶解豆粕分别替代饲料中0(FM组)、25%(R25组)、50%(R50组)、75%(R75组)以及100%的鱼粉(R100组)(鱼粉蛋白与发酵酶解豆粕蛋白配比分别为1:0、3:1、1:1、1:3和0:1),对平均体重为(0.1030±0.0002)g的日本沼虾进行8周的饲养试验,随后对各组虾进行嗜水气单胞菌感染试验。每组设5个重复,每个重复50尾虾。结果表明:当鱼粉蛋白与发酵酶解豆粕蛋白配比为1:1时,日本沼虾的增重率达到最大,显著高于FM和R100组(P<0.05),各组日本沼虾的存活率无显著差异(P>0.05);各组日本沼虾的肝胰腺超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力及丙二醛(MDA)含量无显著差异(P>0.05);FM组日本沼虾的血细胞总数及血淋巴吞噬活性均显著高于R100组(P<0.05);肝胰腺中热应激同源蛋白70和热应激蛋白90的mRNA相对表达水平分别在FM和R25组达到最高,显著高于其余各组(P<0.05);当发酵酶解豆粕替代鱼粉比例超过50%时,嗜水气单胞菌感染后日本沼虾的累计死亡率显著增加(P<0.05)。由此可见,发酵酶解豆粕可作为日本沼虾饲料中较好的蛋白质源,在氨基酸平衡条件下鱼粉蛋白和发酵酶解豆粕蛋白的最佳配比为1:1。

References

[1]  AMAYA E A,DAVIS D A,ROUSE D B.Replacement of fish meal in practical diets for the Pacific white shrimp (Litopenaeus vannamei) reared under pond conditions[J].Aquaculture,2007,262(2/3/4):393-401.
[2]  张凌燕,叶金云,王友慧,等.配合饲料中不同蛋白质水平对日本沼虾生长的影响[J].上海水产大学学报,2008,17(6):668-673.
[3]  秦钦,蔡永祥,陈校辉,等.不同规格日本沼虾饲料蛋白最适含量研究[J].饲料研究,2010(4):53-55.
[4]  陈立侨,堵南山,赖伟.中华绒螯蟹蟹种配饵中豆饼替代部分鱼粉的适宜含量[J].水产学报,1994,18(1):24-31.
[5]  黄峰,严安生,牟松.水产动物饵料诱食剂及其应用[J].中国饲料,1998(14):13-14.
[6]  JOHANSSON M W,KEYSER P,SRITUNYALUCKSANA K,et al.Crustacean haemocytes and haematopoiesis[J].Aquaculture,2000,191(1/2/3):45-52.
[7]  MARER M P,BREHMER D,G SSLER C S,et al.Hsp70 chaperone machines[J].Advances in Protein Chemistry,2001,59(1):1-44.
[8]  BOUTET I,TANGUY A,ROUSSEAU S,et al.Molecular identification and expression of heat shock cognate 70 (hsc70) and heat shock protein 70 (hsp70) genes in the Pacific oyster Crassostrea gigas[J].Cell Stress Chaperones,2003,8(1):76-85.
[9]  OLSVIK P A,TORSTENSEN B E,HEMRE G I,et al.Hepatic oxidative stress in Atlantic salmon (Salmo salar L.) transferred from a diet based on marine feed ingredients to a diet based on plant ingredients[J].Aquaculture Nutrition,2011,17(2):e424-e436.
[10]  沈锦玉,钱冬,刘问,等.养殖青虾"红鳃病"病原的研究[J].浙江海洋学院学报:自然科学版,2000,19(3):222-224.
[11]  FRANCIS G,MAKKAR H P S,BECKER K.Antinutritional factors present in plant-derived alternate fish feed ingredients and their effects in fish[J].Aquaculture,2001,199(3/4):197-227.
[12]  EGOUNLETY M,AWORH O C.Effect of soaking,dehulling,cooking and fermentation with Rhizopus oligosporus on the oligosaccharides,trypsin inhibitor,phytic acid and tannins of soybean (Glycine max Merr.),cowpea (Vigna unguiculata L.Walp) and groundbean (Macrotyloma geocarpa Harms)[J].Journal of Food Engineering,2003,56 (2/3):249-254.
[13]  范彦令,张士辉.酶解豆粕和发酵豆粕的研究进展[J].今日畜牧兽医,2007(7):55-57.
[14]  LIU X,FENG J,XU Z R,et al.The effects of fermented soybean meal on growth performance and immune characteristics in weaned piglets[J].Turkish Journal of Veterinary and Animal Sciences,2007,31(5):341-345.
[15]  YAMAMOTO T,IWASHITA Y,MATSUNARI H,et al.Influence of fermentation conditions for soybean meal in a non-fish meal diet on the growth performance and physiological condition of rainbow trout Oncorhynchus mykiss[J].Aquaculture,2010,309(1/2/3/4):173-180.
[16]  罗智,刘永坚,麦康森,等.石斑鱼配合饲料中发酵豆粕和豆粕部分替代白鱼粉的研究[J].水产学报,2004,28(2):175-181.
[17]  候鑫,梁桂英,阳会军,等.杂交罗非鱼饲料中豆粕、发酵豆粕和晶体氨基酸替代鱼粉的研究[J].南方水产,2009,5(2):28-33.
[18]  KARDER M A,KOSHIO S,ISHIKAWA M,et al.Can fermented soybean meal and squid by-product blend be used as fishmeal replacements for Japanese flounder (Paralichthys olivaceus)?[J].Aquaculture Research,2011,43(10):1427-1438.
[19]  冷向军,王文龙,李小勤.发酵豆粕部分替代鱼粉对凡纳滨对虾的影响[J].粮食与饲料工业,2007(3):40-41.
[20]  MEUNPOL O,RUANGPAN L,VALLISUT S.Replacement of soybean meal protein in fish meal diet in organic marine shrimp feed[J].Asian Journal of Food and Agro-Industry,2009(Suppl.):S175-S181.
[21]  叶倩.发酵豆粕深度酶解生产低分子大豆多肽[D].硕士学位论文.武汉:华中农业大学,2009.
[22]  刘海燕,闫晓刚,邱玉朗,等.发酵酶解豆粕对小鼠血液抗氧化指标和胃肠道消化酶活性及肠道菌群的影响[J].中国畜牧杂志,2011,47(21):41-44.
[23]  刘海燕,邱玉朗,魏炳栋,等.发酵酶解豆粕对仔猪生长性能、日粮营养物质消化率和血液指标的影响[J].吉林农业大学学报,2012,34(6):655-650,666.
[24]  胡亮,薛敏,王彬,等.晶体氨基酸提高混合动物蛋白替代花鲈饲料中鱼粉的潜力[J].水产学报,2011,35(2):268-275.
[25]  FLORETO E A T,BAYER R C,BROWN P B.The effects of soybean-based diets,with and without amino acid supplementation,on growth and biochemical composition of juvenile American lobster,Homarus americanus[J].Aquaculture,2000,189(3/4):211-235.
[26]  ALAM M S,TESHIMA S,KOSHIO S,et al.Supplemental effects of coated methionine and/or lysine to soy protein isolate diet for juvenile kuruma shrimp,Marsupenaeus japonicas[J].Aquaculture,2005,248(1/2/3/4):13-19.
[27]  虞冰如,沈竑.日本沼虾饲料最适蛋白质、脂肪含量及能量蛋白比的研究[J].水产学报,1990,14(4):321-327.
[28]  胡盼,黄旭雄,郭腾飞,等.玉米蛋白粉部分替代鱼粉对日本沼虾生长和肌肉组成的影响[J].上海海洋大学学报,2011,2(2):230-237.
[29]  张琴.刺参(Apostichopus japonicus Selenka)高效免疫增强剂的筛选与应用[D].博士学位论文.青岛:中国海洋大学,2010.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133