全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

饲料中大豆油替代鱼油对大西洋鲑肝细胞脂肪酸代谢的影响

DOI: 10.3969/j.issn.1006-267x.2013.11.015

Keywords: 大豆油,鱼油,大西洋鲑,肝细胞,脂肪酸代谢

Full-Text   Cite this paper   Add to My Lib

Abstract:

为探讨大豆油替代鱼油对大西洋鲑(SalmosalarL.)肝细胞脂肪酸代谢的影响,本试验分别以鱼油和大豆油为脂肪源配制2种试验饲料,并分别饲喂大西洋鲑幼鲑5个月。饲养结束后,分离和培养大西洋鲑肝细胞,一方面将细胞与碳14标记油酸([1-14C]OA)共孵育,然后测定细胞和细胞内各脂质成分以及脂肪酸β-氧化产物的放射活性;另一方面提取肝细胞总RNA,采用实时荧光定量聚合酶链式反应(RT-qPCR)测定脂肪酸结合蛋白3(FABP3)、脂肪酸结合蛋白10(FABP10)、Δ5去饱和酶(D5D)、Δ6去饱和酶(D6D)及肉碱棕榈酰转移酶Ⅰ(CPT-Ⅰ)基因的相对表达量。结果表明:1)鱼油组与大豆油组肝细胞内游离脂肪酸(FFA)放射活性百分率分别为(62.7±13.0)%和(55.9±9.1)%,2组间无显著差异(P>0.05);鱼油组与大豆油组肝细胞内磷脂(PLs)放射活性百分率分别为(20.9±8.7)%和(17.9±5.4)%,2组间无显著差异(P>0.05);鱼油组肝细胞内三酰甘油(TAG)放射活性百分率为(2.0±0.9)%,显著高于大豆油组的(0.8±0.4)%(P<0.05);鱼油组与大豆油组肝细胞内β-氧化产物放射活性百分率分别为(1.0±0.3)%和(1.0±0.2)%,2组间无显著差异(P>0.05)。2)大豆油组肝细胞中FABP3、D6D、D5D基因的相对表达量均显著高于鱼油组(P<0.05),而FABP10和CPT-Ⅰ基因的相对表达量在2组间无显著差异(P>0.05)。由此可见,大豆油替代饲料中的鱼油对大西洋鲑肝细胞内FFA、PLs合成及脂肪酸β-氧化等脂肪酸代谢途径无显著影响,但显著降低了肝细胞内TAG的合成,促进了肝细胞内脂肪酸的去饱和,因此大豆油替代鱼油在一定程度上影响了肝细胞内脂肪酸代谢。

References

[1]  BARLOW S.Fishmeal and fish oil:sustainable feed ingredients for aquafeeds[J].Global Aquacult Advocate,2000,4:85-88.
[2]  SARGENT J R,BELL J G,MCEVOY L,et al.Recent developments in the essential fatty acid nutrition of fish[J].Aquaculture,1999,177(1/2/3/4):191-199.
[3]  BELL J G,MCEVOY J,TOCHER D R,et al.Replacement of fish oil with rapeseed oil in diets of Atlantic salmon(Salmo salar)affects tissue lipid compositions and hepatocyte fatty acid metabolism[J].The Journal of Nutrition,2001,131(5):1535-1543.
[4]  BELL J G,HENDERSON R J,TOCHER D R,et al.Substituting fish oil with crude palm oil in the diet of Atlantic salmon (Salmo salar) affects muscle fatty acid composition and hepatic fatty acid metabolism[J].The Journal of Nutrition,2002,132(2):222-230.
[5]  BELL J G,TOCHER D R,HENDERSON R J,et al.Altered fatty acid compositions in Atlantic salmon (Salmo salar) fed diets containing linseed and rapeseed oils can be partially restored by a subsequent fish oil finishing diet[J].The Journal of Nutrition,2003,133(9):2793-2801.
[6]  BRANSDEN M P,CARTER C G,NICHOLS P D.Replacement of fish oil with sunflower oil in feeds for Atlantic salmon (Salmo salar L):effect on growth performance,tissue fatty acid composition and disease resistance[J].Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,2003,135(4):611-625.
[7]  DANNEVIG B H,BERG T.Endocytosis of galactose-terminated glycoproteins by isolated liver cells of the rainbow trout (Salmo gairdneri)[J].Comparative Biochemistry and Physiology B:Comparative Biochemistry,1985,82(4):683-688.
[8]  FOLCH J,LEES M,SLOANE STANLEY G H.A simple method for the isolation and purification of total lipides from animal tissues[J].Journal of Biology and Chemistry,1957,226(1):497-509.
[9]  TELLMAN G.The E-Method:a highly accurate technique for gene-expression analysis[J].National Methods,2006,3(7):i-ii.doi:10.1038/nmeth894.
[10]  VANDESOMPELE J,DE PRETER K,PATTYN F,et al.Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes[J].Genome Biology,2002,3(7):research0034.1-0034.11.
[11]  LEAVER M J,BAUTISTA J M,BJOMSSON B T,et al.Towards fish lipid nutrigenomics:current state and prospects for fin-fish aquaculture[J].Fisheries Science,2008,16(Suppl.1):71-92.
[12]  GREGORY M K,KING H W,BAIN P A,et al.Development of a fish cell culture model to investigate the impact of fish oil replacement on lipid peroxidation[J].Lipids,2011,46(8):753-764
[13]  SMALL G,CONNOCK M.Palmitoyl-CoA oxidase in goldfish (Carassius auratus):detection in several tissues and subcellular location in intestinal peroxisomes[J].Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,1981,68:151-153.
[14]  MANNAERTS G P,DEBEER L J,THOMAS J,et al.Mitochondrial and peroxisomal fatty acid oxidation in liver homogenates and isolated hepatocytes from control and clofibrate-treated rats[J].Journal of Biology and Chemistry,1979,254(11):4585-4595.
[15]  FOERSTER E C,FAHRENKEMPER F,RABE U,et al.Peroxisomal fatty acid oxidation as detected by H2O2 production in intact perfused rat liver[J].Journal of Biochemistry,1981,196(3):705-712.
[16]  POWER G W,NEWSHOLME E A.Dietary fatty acids influence the activity and metabolic control of mitochondrial carnitine palmitoyltransferase Ⅰ in rat heart and skeletal muscle[J].The Journal of Nutrition,1997,127(11):2142-2150.
[17]  VEERKAMP J H,VANMOERKERK H T B.Fatty acid-binding protein and its relation to fatty acid oxidation[J].Molecular and Cellular Biochemistry,1993,123(1/2):101-106.
[18]  FURUHASHI M,URA N,MURAKAMI H,et al.Fenofibrate improves insulin sensitivity by reducing muscle lipid content via increase of fatty acid binding protein and β-oxidation in muscle[J].Journal of Hypertens,2002,20:S56.
[19]  KO Y H,CHENG C H,SHEN T F,et al.Cloning and expression of Tsaiya duck liver fatty acid binding protein[J].Poultry Science,2004,83(11):1832-1838.
[20]  DI PIETRO S M,DELL’ANGELICA E C,SCHLEICHER C H,et al.Purification and structural characterization of a fatty acid-binding protein from the liver of the catfish Rhamdia sapo[J].Comparative of Biochemistry and Physiology Part B:Biochemistry & Molecular Biology,1996,113(3):503-509.
[21]  ZHENG X Z,TOCHER D R,DICKSON C A,et al.Effects of diets containing vegetable oil on expression of genes involved in highly unsaturated fatty acid biosynthesis in liver of Atlantic salmon (Salmo salar)[J].Aquaculture,2004,236(1/2/3/4):467-483.
[22]  JORDAL A E,TORSTENSEN B E,TSOI S,et al.Dietary rapeseed oil affects the expression of genes involved in hepatic lipid metabolism in Atlantic salmon (Salmo salar L.)[J].The Journal of Nutrition,2005,135(10):2355-2361.
[23]  BRENNER R R.Nutritional and hormonal factors influencing desaturation of essential fatty acids[J].Progress in Lipid Research,1981,20:41-47.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133