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十二指肠灌注亮氨酸对奶牛胰腺淀粉酶分泌的影响

DOI: 10.3969/j.issn.1006-267x.2013.08.016

Keywords: 亮氨酸,十二指肠灌注,淀粉酶,胰岛素,胆囊收缩素

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Abstract:

本试验旨在研究十二指肠灌注亮氨酸对奶牛胰腺淀粉酶分泌的影响.以4头周岁荷斯坦母牛[(215±7)kg]为试验动物,手术安装十二指肠胰液收集袋、灌注管和回流管以及颈静脉插管,采用4×4拉丁方试验设计,进行十二指肠亮氨酸灌注试验.结果表明,随亮氨酸灌注水平升高,血浆胰岛素和胆囊收缩素浓度升高(P<0.05),胰腺α-淀粉酶合成速率(U/h)呈先升高后降低趋势(P<0.01),在29.5μmol/(kg·h)灌注水平时最大.十二指肠灌注亮氨酸也极显著影响了胰腺α-淀粉酶分泌浓度(U/L,P<0.01;U/gprot,P<0.01).结果表明,亮氨酸可能通过刺激胰岛素和胆囊收缩素释放,调控青年奶牛胰腺淀粉酶分泌功能,二者存在剂量效应.

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