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不同氨基酸模式对奶牛乳腺上皮细胞酪蛋白合成的影响

DOI: 10.3969/j.issn.1006-267x.2013.08.013

Keywords: 乳腺上皮细胞,氨基酸模式,基因表达,酪蛋白

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Abstract:

本试验旨在验证氨基酸模式的不同是否会影响奶牛乳腺上皮细胞酪蛋白的合成.采用完全随机试验设计设置4个组,分别为低蛋白质饲粮条件下血液氨基酸模式组(LPBP)、全乳蛋白氨基酸模式组(MP)、80%酪蛋白+20%乳清蛋白氨基酸模式组(CLP)、酪蛋白氨基酸模式组(CP),每组3个重复,并重复试验3次.分别用实时荧光定量PCR(RT-qPCR)和酶联免疫吸附法(ELISA)检测乳蛋白合成基因αs1-酪蛋白(CSN1S1)和κ-酪蛋白(CSN3)mRNA表达量及αs-酪蛋白合成量.结果表明:氨基酸模式能够显著影响CSN1S1和CSN3基因mRNA表达量(P<0.05).MP组CSN1S1和CSN3基因mRNA表达量分别显著和极显著高于LPBP组(P<0.05和P<0.01);相对于LPBP组,MP组CSN1S1和CSN3基因mRNA表达量分别上调了1.70和2.12倍.MP组αs-酪蛋白合成量显著高于CLP、CP组(P<0.05),极显著高于LPBP组(P<0.01).由此可见,氨基酸模式的不同能够影响奶牛乳腺上皮细胞酪蛋白的合成,全乳蛋白氨基酸模式可能是一种较为理想的氨基酸模式.

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