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饲粮能氮限饲与补偿对蒙古羔羊肝脏重量、肝细胞增殖和增肥及生长激素受体、类胰岛素生长因子基因表达量的影响

DOI: 10.3969/j.issn.1006-267x.2013.07.030

Keywords: 能氮限饲,能氮补偿,蒙古羔羊,肝细胞增殖,肝细胞增肥,GHR,IGF-Ⅰ,IGF-Ⅱ,基因表达量

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Abstract:

本文旨在研究饲粮能氮限饲与补偿对蒙古羔羊肝脏重量、肝细胞增殖和增肥及生长激素受体(GHR)、类胰岛素生长因子Ⅰ(IGF-Ⅰ)、类胰岛素生长因子Ⅱ(IGF-Ⅱ)基因表达量的影响。选用体重相近的蒙古羔羊80只,随机分为4组,分别为对照组、蛋白质限饲组(PR组)、能量限饲组(ER组)、蛋白质能量双重限饲组(BR组),限饲期(60d)内各组分别饲喂粗蛋白质(CP)为15.08%、9.24%、15.01%和6.27%,对应代谢能(ME)为10.61、10.49、8.36和8.10MJ/kg的饲粮。之后的90d补偿期,各组均饲喂相同营养水平(CP为12.48%、ME为9.77MJ/kg)的饲粮。分别于限饲期和补偿期结束时屠宰,采集肝脏组织,称重,测定肝脏DNA含量、肝细胞DNA和蛋白质浓度,并应用实时定量PCR技术检测GHR、IGF-Ⅰ和IGF-Ⅱ基因表达量的变化。结果表明:限饲60d后,ER组(P<0.05)、BR组(P<0.01)羔羊肝脏重量显著或极显著低于对照组;PR组、ER组和BR组肝细胞数量均低于对照组,肝细胞体积变化不大;各限饲组羔羊肝脏IGF-Ⅰ基因表达量显著低于对照组(P<0.05);PR组(P<0.01)、ER组(P<0.01)和BR组(P<0.05)羔羊肝脏IGF-Ⅱ基因表达量显著或极显著低于对照组。补偿90d后,各限饲组羔羊肝脏重量、肝细胞数量、肝细胞体积以及GHR、IGF-Ⅰ和IGF-Ⅱ基因表达量与对照组均无显著差异(P>0.05)。结果提示,3月龄开始限饲的蒙古羔羊,限饲能量对肝脏生长发育的抑制强于比限饲蛋白质,而蛋白质能量双重限饲造成的负面影响最大;单独限饲能量或蛋白质羔羊肝脏中GHR、IGF-Ⅰ和IGF-Ⅱ基因表达量的下降幅度大于蛋白质能量双重限饲;经过饲粮能氮补偿,限饲影响的指标均恢复到与未限饲相同水平。

References

[1]  李东,考桂兰,侯先志,营养限饲和补偿对羔羊消化道器官质量的影响[J].饲料研究,2011(11):59-60.
[2]  李东,考桂兰,侯先志,营养限饲和补偿对羔羊体质量和内脏质量的影响[J].饲料研究,2011(3):12-15.
[3]  KAMALZADEH A,KOOPS W J,VAN BRUCHEM J,et al.Feed quality restriction and compensatory growth in growing sheep:development of body organs[J].Small Ruminant Research,1998,29:71-82.
[4]  GóMEZ-PASTéN M,MORA O,PEDRAZA-CHAVERRI J,et al.The effect of a long term feed restriction on metabolism and tissue composition of goats[J].Agricultural Science,1999,132:227-232.
[5]  KAMALZADEHL A,KOOPS W J,KIASAT A,et al.Effect of qualitative feed restriction and nitrogen retention energy metabolism sheep[J].South African Journal of Animal Science,2009,39(1):30-39.
[6]  THISSEN J P,KETELSLEGERS J M,UNDER-WOOD L E.Nutritional regulation of the insulin-like growth factors[J].Endocrine Reviews,1994,15(1):80-101.
[7]  BILAL T,KESER O,SERPEK B,et al.The Effects of dietary protein level on serum growth hormone,insulin-like growth factors and performance in Kivircik lambs[J].Medwell Journals,2008(7):1383-1386.
[8]  杨美霞.限饲及补偿生长对母羊器官组织中生长轴激素表达水平的影响.硕士学位论文.呼和浩特:内蒙古农业大学,2007.
[9]  刘小刚,营养限饲及补偿对羔羊内脏器官及血液中CD4+和CD8+ T淋巴细胞的影响.硕士学位论文.呼和浩特:内蒙古农业大学,2010.
[10]  高峰.妊娠后期限饲母羊对其胎儿生长发育及出生后羔羊补偿生长的影响.博士学位论文.呼和浩特:内蒙古农业大学,2006.
[11]  FRENCH M C,LITTLEJOHN R P,GREER G J,et al.Growth hormone and ghrelin receptor genes are differentially expressed between genetically lean and fat selection lines of sheep[J].Animal Science,2006,84:324-331.
[12]  MACLAUGHLIN S M,WALKER S K,KLEEMANN D O,et al.Impact of periconceptional undernutrition on adrenal growth and adrenal insulin-like growth factor and steroidogenic enzyme expression in the sheep fetus during early pregnancy[J].Endocrinology,2007,148(4):1911-1920.
[13]  余舜武,刘鸿艳,罗利军,等.利用不同实时定量PCR方法分析相对基因表达差异[J].作物学报,2007(7):1214-1218.
[14]  LIVAK K J,SCHMITTGEN T D.Analysis of relative gene expression data using real time quantitative PCR and the 2-ΔΔCt method[J].Methods,2001,25(4):402-408.
[15]  齐景伟,侯先志,杨美霞,等.营养限饲和补偿生长对妊娠期蒙古绵羊生长激素受体(GHR)mRNA表达水平的影响[J].黑龙江畜牧兽医,2007(3):4-6.
[16]  EL KHATTABI I,GRéGOIRE F,REMACLE C,et al.Isocaloric maternal low-protein diet alters IGF-Ⅰ,IGFBPs,and hepatocyte proliferation in the fetal rat[J].Endocrinology Metabolism,2003,285:E991-E1000.
[17]  HYATT M A,GOPALAKRISHNAN G S,BISPHAM J,et al.Maternal nutrient restriction in early pregnancy programs hepatic mRNA expression of growth-related genes and liver size in adult male sheep[J].Endocrinology,2007,192:87-97.
[18]  RADCLIFF R P,VANDEHAAR M J,KOBAYASHI Y,et al.Effect of dietary energy and somatotropin on components of the somatotropic axis in Holstein heifers[J].Journal of Dairy Science,2004,87(5):1229-1235.
[19]  BRAMELD J M,GILMOUR R S,BUTTERY P J,et al.Glucose and amino acid interaction with hormone to control expression of insulin-like factor-1 and growth hormone receptor mRNA in cultured pig hepatocytes[J].The Journal of Nutrition,1999,129:1298-1306.
[20]  李俊良,侯先志,杨金丽,等.营养限制与补偿对蒙古羔羊体重和血液中生长相关激素的影响[J].饲料工业,2012,33(9):43-47.
[21]  周其伟,白春生,贾斌,等.中国美利奴绵羊肉用品系生长轴相关基因表达对其十二指肠发育的影响[J].黑龙江兽医学报,2010,41(8):967-973.
[22]  MARTIN M A,SERRADAS P,RAMOS S,et al.Protein-caloric food restriction affects insulin-like growth factor system in fetal Wistar rat[J].Endocrinology,2005,146(3):1364-1371.
[23]  程胜利,李建升,冯瑞林,等.赖氨酸对绵羊肝脏和背最长肌中GHR和IGF-Ⅰ基因表达调控的影响[J].黑龙江畜牧兽医,2011(3):1-4.
[24]  GONG L,PAN Y X,CHEN H,et al.Gestational low protein diet in the rat mediates IgF2 gene expression in male of spring via altered hepatic DNA methylation[J].Epigenetics,2010,5(7):619-626.
[25]  徐永霞,侯先志,杨金丽,等.营养限饲与补偿对蒙古育成羯羊肝脏关键酶活性的影响[J].动物营养学报,2012,24(7):1236-1245.
[26]  刘国庆,黄治国,刘振山,等.羔羊肝脏IGF-Ⅰ和IGF-ⅠR基因表达的发育性变化研究[J].中国农业科学,2006,39(12):2577-2581.

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