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甘露聚糖酶对罗非鱼生长性能、消化代谢和非特异性免疫力的影响

DOI: 10.3969/j.issn.1006-267x.2013.07.031

Keywords: 甘露聚糖酶,罗非鱼,生长性能,消化代谢,非特异性免疫力

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Abstract:

本试验旨在研究饲料中添加甘露聚糖酶对罗非鱼生长性能、消化代谢和非特异性免疫力的影响。在基础饲料中分别添加0(对照)、500、1000U/kg的甘露聚糖酶,配制3种等氮(粗蛋白质水平为33.5%)等能(总能为15.7MJ/kg)的试验饲料,分别投喂平均体重为(5.80±0.01)g的吉富罗非鱼(GIFT,Oreochromisniloticus)8周。每组设3个重复,每个重复30尾鱼。结果表明:1000U/kg组罗非鱼的末重、特定生长率(SGR)和蛋白质效率(PER)显著高于其他组(P<0.05)。各组罗非鱼的饲料系数(FCR)、摄食量(FI)、成活率(SR)差异不显著(P>0.05)。随饲料中甘露聚糖酶添加水平的增加,罗非鱼肠道淀粉酶、胰蛋白酶、Na+,K+-ATP酶的活性显著升高(P<0.05)。1000U/kg组罗非鱼血液谷草转氨酶(AST)活性和高密度脂蛋白胆固醇(HDL-C)含量显著低于对照组和500U/kg组(P<0.05),而总胆固醇(TC)、极低密度脂蛋白胆固醇(VLDL-C)含量却显著高于对照组和500U/kg组(P<0.05)。各组罗非鱼血液谷丙转氨酶(ALT)活性、甘油三酯(TG)含量、低密度脂蛋白胆固醇(LDL-C)含量和白细胞总数以及血清总超氧化物歧化酶(T-SOD)活性均无显著差异(P>0.05)。500和1000U/kg组罗非鱼血液中性粒细胞、单核细胞百分比以及血清碱性磷酸酶(AKP)和过氧化氢酶(CAT)活性均显著高于对照组(P<0.05),而淋巴细胞百分比却显著低于对照组(P<0.05)。由此可见,饲料中添加适宜水平的甘露聚糖酶可以提高罗非鱼的消化吸收能力,增强非特异性免疫力,进而促进其生长。

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