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稀释率对新型固液气分流式瘤胃模拟系统发酵效果的影响

DOI: 10.3969/j.issn.1006-267x.2013.07.018

Keywords: 稀释率,固液气分流式瘤胃模拟系统,瘤胃发酵

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Abstract:

本试验旨在研究稀释率对新型固液气分流式瘤胃模拟系统发酵效果的影响。采用单因子完全随机试验设计,共设4个处理,各处理稀释率分别为6%/h、8%/h、10%/h、12%/h,每个处理3个重复,每个重复1个发酵罐。试验期10d。结果表明:随着稀释率的提高,发酵液pH极显著提高(P<0.01),而氨态氮(NH3-N)浓度则极显著降低(P<0.01)。稀释率由6%/h上升到10%/h,总挥发性脂肪酸(TVFA)及戊酸浓度极显著下降(P<0.01)。稀释率从6%/h提高到8%/h时,原虫数量极显著提高了6.91%(P<0.01),但当稀释率从8%/h提高到12%/h时,原虫数量却极显著下降了5.18%(P<0.01)。随着稀释率从6%/h增加到10%/h,木聚糖酶活性(P<0.01)及微生物蛋白质(MCP)产量(P<0.05)显著或极显著降低,粗蛋白质体外消失率极显著升高(P<0.01)。稀释率为10%/h和12%/h的2个处理,中性洗涤纤维体外消失率显著高于另外2个处理(P<0.05)。结果提示,稀释率能够影响新型固液气分流式瘤胃模拟系统的发酵情况,稀释率为8%/h时,原虫数量以及纤维素类酶活性、各瘤胃发酵参数更加有利于发酵,因此可推荐作为本系统最优运行条件。

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