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乙酸浓度对奶牛乳腺上皮细胞甘油三酯含量及瘦素和过氧化物酶增殖物激活受体γ基因表达量的影响

DOI: 10.3969/j.issn.1006-267x.2013.07.016

Keywords: 乙酸,奶牛乳腺上皮细胞,瘦素,过氧化物酶增殖物激活受体γ

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Abstract:

本试验旨在研究乙酸浓度对奶牛乳腺上皮细胞甘油三酯(TAG)含量及瘦素(leptin)和过氧化物酶增殖物激活受体γ(PPARγ)基因表达量的影响。将传至第3代的奶牛乳腺上皮细胞以1×105个/孔的密度培养48h后,随机分为6个处理,各处理分别采用乙酸浓度为0(对照组)、4、6、8、10和12mmol/L的培养液。置于37℃5%CO2培养箱继续培养48h。收集细胞,观测脂滴形成状况,测定TAG含量及leptin和PPARγ基因表达量。结果表明:随乙酸浓度的增加,培养的奶牛脂肪前体细胞脂滴形成增多;随乙酸浓度的增加,TAG含量和PPARγ基因表达量均呈显著的线性或二次曲线增加(P<0.05),且以乙酸浓度为10和12mmol/L时效果较好;一定浓度的乙酸可上调leptin基因表达量,尤以浓度为8、10mmol/L时上调作用较好。结果提示,乙酸对奶牛乳腺上皮细胞内脂滴的形成、TAG的积累、leptin和PPARγ基因的表达有显著的促进作用。本试验条件下,乙酸浓度为10~12mmol/L时能较好地促进PPARγ基因的表达,浓度为8~10mmol/L时能较好地促进leptin基因的表达。

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