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鸡β-防御素-9的融合表达及其多克隆抗体的制备与鉴定

DOI: 10.3969/j.issn.1006-267x.2013.03.019

Keywords: 鸡β-防御素-9,抗菌肽,融合蛋白,多克隆抗体

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Abstract:

为制备鸡β-防御素-9(AvBD9)多克隆抗体,本试验将AvBD9基因插入pGEX-6P-1载体,构建重组基因pGEX6P-AvBD9大肠杆菌表达质粒。将通过切胶纯化获得的表达产物包涵体谷胱甘肽转移酶-AvBD9(GST-AvBD9)融合蛋白免疫新西兰大白兔,制备抗AvBD9血清,并检测抗体效价。结果表明,经间接酶联免疫分析(ELISA)及蛋白质免疫印迹(Westernblot)方法证实,获得的融合蛋白免疫新西兰大白兔得到了特异性的多克隆抗体,抗体效价为1:38400。

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