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板栗总苞多酚提取工艺优化及其抗氧化性研究

DOI: 10.3969/j.issn.1006-267x.2013.02.023

Keywords: 板栗总苞,多酚,提取工艺,抗氧化性

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Abstract:

本试验旨在探索提取板栗总苞多酚的最优工艺条件及研究其体外抗氧化能力。在单因素试验设计得出的提取条件的基础上,设计正交试验,确定最优提取工艺条件,并分别通过二苯代苦味酰基自由基(DPPH·)和2,2'-连氮-双-(3-乙基苯并噻唑啉-6-磺酸)自由基(ABTS+·)清除率以及猪油过氧化值(POV)的测定对板栗总苞多酚进行体外抗氧化性研究。结果表明:1)最优提取工艺条件:乙醇浓度为30%,液料比为18:1(mL:g),提取时间为140min,提取温度为80℃。采用该工艺条件,板栗总苞多酚提取得率为22.61%,其中多酚含量为51.23%。2)板栗总苞多酚对DPPH·和ABTS+·清除率与维生素C相当,且均大于维生素E;板栗总苞多酚对猪油有良好的抗氧化效果,抗氧化能力与板栗总苞多酚的浓度有关,100μg/mg为板栗总苞多酚对猪油抗氧化的最佳浓度,此浓度下板栗总苞多酚的抗氧化效果与同浓度的维生素C相当,且均优于同浓度的维生素E。因此,在本试验最优提取工艺条件下,板栗总苞多酚提取得率和多酚含量较高,且得到的板栗总苞多酚有良好的抗氧化能力。

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