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爱拔益加肉仔鸡肠道PepT1、B0AT和EAAT3mRNA的表达差异与发育规律

DOI: 10.3969/j.issn.1006-267x.2014.09.048

Keywords: 肉仔鸡,肠道,PepT1,氨基酸转运载体,发育变化

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Abstract:

本试验旨在研究爱拔益加(AA)肉仔鸡肠道小肽转运载体1(PepT1)、B0系统中性氨基酸转运载体(B0AT)、兴奋性氨基酸转运载体3(EAAT3)mRNA的表达差异与发育规律。试验选取1日龄雄性AA肉仔鸡120只,随机分为8个组,于1、7、14、21、28、35、42日龄,每个组选取1只肉仔鸡,采用实时荧光定量PCR技术检测不同肠段PepT1、B0AT、EAAT3mRNA的相对表达量。结果显示:1)肉仔鸡不同肠段PepT1、B0AT、EAAT3mRNA的相对表达量差异显著(P<0.05)。其中,PepT1mRNA的相对表达量在十二指肠最高,空肠次之,回肠最低,B0AT、EAAT3mRNA的相对表达量在回肠最高,空肠次之,十二指肠最低。2)肉仔鸡不同日龄PepT1、B0AT、EAAT3mRNA的相对表达量存在极差异显著(P<0.01);在不同肠段上,PepT1、B0AT、EAAT3mRNA的相对表达量随日龄的变化均表现为前期升高,后期平稳的趋势。由此可见,肉仔鸡肠道参与小肽及氨基酸吸收的转运载体PepT1、B0AT、EAAT3mRNA表达具有肠段及日龄的差异性。

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