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黑曲霉固态发酵改善木薯渣品质的研究

DOI: 10.3969/j.issn.1006-267x.2014.07.039

Keywords: 黑曲霉,固态发酵,木薯渣,营养价值

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Abstract:

本试验旨在研究黑曲霉固态发酵对木薯渣营养价值的影响,并探讨最佳的木薯渣发酵工艺参数。先采用单因素试验设计,根据发酵后木薯渣的营养价值,分别筛选出最佳的黑曲霉接种量、菜籽粕添加量、发酵时间、发酵温度、料水比以及初始pH。在此基础上采用正交试验设计,考察发酵时间、发酵温度、料水比以及初始pH间交互作用对木薯渣营养价值的影响。结果表明:1)每克发酵原料接种3×106个黑曲霉孢子,添加15%的菜籽粕,料水比1:1.50,初始pH4,在36℃条件下发酵4d效果最好。2)最优条件发酵后,与对照组相比,以干物质为基础,粗纤维含量由22.26%降低到17.71%(P<0.05),还原糖含量由1.42%提高到5.11%(P<0.05),粗蛋白质含量由8.67%提高到13.48%(P<0.05),粗脂肪含量由7.89%提高到11.72%(P<0.05),粗灰分含量由6.02%提高到7.31%(P<0.05);此外,发酵后羧甲基纤维素酶活性达到9.05U/g,滤纸酶活性达到2.37U/g,β-葡萄糖苷酶活性达到2.25U/g。由此可见,利用黑曲霉固态发酵可以显著提高木薯渣的营养价值。

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