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海洋通报  2011 

长牡蛎基因组微卫星引物的开发和特性描述

DOI: 10.11840/j.issn.1001-6392.2011.5.015, PP. 566-571

Keywords: 长牡蛎,微卫星,标记开发,基因组

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Abstract:

长牡蛎于20世纪80年代从日本引进中国。在我国,长牡蛎已经成为重要的贝类养殖产业之一。本实验从全基因组上筛查微卫星序列,在微卫星筛查的范围、数目和类型上是传统的富集文库法开发微卫星所无法比拟的。利用基因组微卫星序列总共设计了104对引物,54对引物能扩增出目的片段,其中有34对引物显示多态性扩增,占32.7%,20对引物显示单态性扩增,占19.2%。在自然群体48个个体样本中分析了这些位点的多态性,结果表明:等位基因数目在2~8之间,观测杂合度和期望杂合度分别在0.0652~0.7955和0.0638~0.8534之间。34对微卫星分子标记中有7对符合哈迪-温伯格平衡,27对或多或少的偏离平衡。微卫星分子标记可以用作分子遗传育种、遗传连锁图谱的构建、种群遗传结构的分析、亲缘关系分析等方面。

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