全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

βCaMKII过量表达损害小鼠海马齿状回区长时程抑制

, PP. 54-62

Keywords: β钙离子/钙调素依赖的蛋白激酶II,海马齿状回,长时程抑制

Full-Text   Cite this paper   Add to My Lib

Abstract:

采用海马齿状回(dentategyrus,DG)区域特异性过量表达βCaMKII的蛋白修饰转基因小鼠,通过离体电生理技术,研究了βCaMKII高表达对该区域突触可塑性的影响.与对照组相比,转基因小鼠海马齿状回双脉冲抑制反应(paired-pulsedepression,PPD)和颗粒细胞的电压—电流曲线(voltage-currentcurve)没有发生变化,而该区域的长时程抑制(long-termdepression,LTD)明显被减弱.实验结果提示,βCaMKII的过量表达不影响小鼠海马齿状回区的突触前递质释放能力和颗粒细胞的被动属性,但损害其长时程抑制.这为进一步研究βCaMKII在学习记忆和突触可塑性中的作用提供了电生理学依据.

References

[1]  O’KEEFE J N L. The Hippocampus as a Cognitive Map[M]. Oxford: Oxford University Press, 1978.
[2]  LANGSTON R F, STEVENSON C H, WILSON C L, et al. The role of hippocampal subregions in memory for stimulus associations[J]. Behav Brain Res, 2010, 215: 275-291.
[3]  TAMMINGA C A, STAN A D, WAGNER A D. The hippocampal formation in schizophrenia[J]. Am J Psychiatry, 2010, 167: 1178-1193.
[4]  HSU D. The dentate gyrus as a filter or gate: a look back and a look ahead[J]. Prog Brain Res, 2007, 163: 601-613.
[5]  SAHAY A, DREW M R, HEN R. Dentate gyrus neurogenesis and depression[J]. Prog Brain Res, 2007, 163: 697-722.
[6]  CAO X, WANG H, MEI B, et al. Inducible and selective erasure of memories in the mouse brain via chemical-genetic manipulation[J]. Neuron, 2008, 60: 353-366.
[7]  MAYFORD M, WANG J, KANDEL E R, et al. CaMKII regulates the frequency-response function of hippocampal synapses for the production of both LTD and LTP[J]. Cell, 1995, 81: 891-904.
[8]  SILVA A J, PAYLOR R, WEHNER J M,et al. Impaired spatial learning in alpha-calcium-calmodulin kinase II mutant mice[J]. Science, 1992, 257: 206-211.
[9]  BENNETT M K, ERONDU N E, KENNEDY M B. Purification and characterization of a calmodulin-dependent protein kinase that is highly concentrated in brain[J]. J Biol Chem, 1983, 258: 12735-12744.
[10]  ERONDU N E, KENNEDY M B. Regional distribution of type II Ca2+/calmodulin-dependent protein kinase in rat brain[J]. J Neurosci, 1985(5): 3270-3277.
[11]  HAYASHI Y, SHI S H, ESTEBAN J A, et al. Driving AMPA receptors into synapses by LTP and CaMKII: requirement for GluR1 and PDZ domain interaction[J]. Science, 2000, 287: 2262-2267.
[12]  PONCER J C, ESTEBAN J A, MALINOW R. Multiple mechanisms for the potentiation of AMPA receptor-mediated transmission by alpha-Ca2+/calmodulin-dependent protein kinase II[J]. J Neurosci, 2002, 22: 4406-4411.
[13]  CHO MH, CAO X, WANG D〖WTBX〗,〖WTBZ〗et al. Dentate gyrus-specific manipulation of beta-Ca2+/calmodulin-dependent kinase II disrupts memory consolidation[J]. Proc Natl Acad Sci U S A, 2007, 104: 16317-16322.
[14]  NICHOLLS R E, ALARCON J M, MALLERET G〖WTBX〗,〖WTBZ〗et al. Transgenic mice lacking NMDAR-dependent LTD exhibit deficits in behavioral flexibility[J]. Neuron, 2008, 58: 104-117.
[15]  TSIEN J Z, HUERTA P T, TONEGAWA S. The essential role of hippocampal CA1 NMDA receptor-dependent synaptic plasticity in spatial memory[J]. Cell, 1996, 87: 1327-1338.
[16]  SCHNABEL R, PALMER M J, KILPATRICK I C, et al. A CaMKII inhibitor, KN-62, facilitates DHPG-induced LTD in the CA1 region of the hippocampus[J]. Neuropharmacology, 1999, 38: 605-608.
[17]  KIRKWOOD A, SILVA A, BEAR M F. Age-dependent decrease of synaptic plasticity in the neocortex of alphaCaMKII mutant mice[J]. Proc Natl Acad Sci U S A, 1997, 94: 3380-3383.
[18]  LAI Y, NAIRN A C, GREENGARD P. Autophosphorylation reversibly regulates the Ca2+/calmodulin-dependence of Ca2+/calmodulin-dependent protein kinase II[J]. Proc Natl Acad Sci USA, 1986, 83: 4253-4257.
[19]  BROCKE L, CHIANG L W, WAGNER P D, et al. Functional implications of the subunit composition of neuronal CaM kinase II[J]. J Biol Chem, 1999, 274: 22713-22722.
[20]  SHEN K, MEYER T. Dynamic control of CaMKII translocation and localization in hippocampal neurons by NMDA receptor stimulation[J]. Science, 1999, 284: 162-166.
[21]  SHEN K, TERUEL M N, CONNOR J H, et al. Molecular memory by reversible translocation of calcium/calmodulin-dependent protein kinase II[J]. Nat Neurosci, 2000(3): 881-886.
[22]  THIAGARAJAN T C, PIEDRAS-RENTERIA E S, TSIEN R W. Alpha- and betaCaMKII. Inverse regulation by neuronal activity and opposing effects on synaptic strength[J]. Neuron, 2002, 36: 1103-1114.
[23]  LEE H K, BARBAROSIE M, KAMEYAMA K, et al. Regulation of distinct AMPA receptor phosphorylation sites during bidirectional synaptic plasticity[J]. Nature, 2000, 405: 955-959.
[24]  DEBANNE D, GUERINEAU N C, GAHWILER B H, et al. Paired-pulse facilitation and depression at unitary synapses in rat hippocampus: quantal fluctuation affects subsequent release[J]. J Physiol, 1996, 491 ( Pt 1): 163-176.
[25]  ZUCKER R S, REGEHR W G. Short-term synaptic plasticity[J]. Annu Rev Physiol, 2002, 64: 355-405.
[26]  MASSEY P V, BASHIR Z I. Long-term depression: multiple forms and implications for brain function[J]. Trends Neurosci, 2007, 30: 176-184.
[27]  KEMP A, MANAHAN-VAUGHAN D. Hippocampal long-term depression: master or minion in declarative memory processes?[J]. Trends Neurosci, 2007, 30: 111-118.
[28]  ZENG H, CHATTARJI S, BARBAROSIE M, et al. Forebrain-specific calcineurin knockout selectively impairs bidirectional synaptic plasticity and working/episodic-like memory[J]. Cell, 2001, 107: 617-629.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133