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聚L-谷氨酸-γ-苄酯纳米粒子提高耐药细胞药物累积的研究

, PP. 141-149

Keywords: 多药耐药性,多聚体药物输送系统,纳米技术,纳米药物

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Abstract:

研究PGG载体在耐药肿瘤细胞中的作用.通过诱导A549细胞产生多药耐药性,评估在耐药肿瘤细胞中PGG对抗癌药物多柔比星胞内累积和驻留的影响.证明PGG药物输送系统能够增加耐药细胞株中的药物累积.利用内吞抑制试验和透射电镜试验阐明细胞通过胞饮方式摄取PGG载体药物,该方式提高了药物的细胞摄取,增加了药物的细胞累积.并利用激光共聚焦显微术确认了药物的细胞累积.PGG-Dox同游离Dox相比,通过胞饮作用进入细胞后,可以显著增加药物处理细胞内的Dox累积和驻留.Dox与PGG药物输送系统连接后,具有显著的抗耐药效果.PGG药物输送系统具备应用于耐药型肿瘤治疗的潜力.

References

[1]  MINOTTI G, MENNA P, SALVATORELLI E, et al. Anthracyclines: molecular advances and pharmacologic developments in antitumor activity and cardiotoxicity[J]. Pharmacological Reviews, 2004,56: 185-229. 
[2]  DAVE B, CHANG J. Treatment resistance in stem cells and breast cancer[J]. Journal of Mammary Gland Biology and Neoplasia, 2009,14: 79-82. 
[3]  BORST P, ELFERINK R O. Mammalian ABC transporters in health and disease[J]. Annual Review of Biochemistry,2002,71: 537-592. 
[4]  BROXTERMAN H J, GOTINK K J, VERHEUL H M. Understanding the causes of multidrug resistance in cancer: A comparison of doxorubicin and sunitinib[J]. Drug Resistance Updates: Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy, 2009(12): 114-126. 
[5]  AMBUDKAR S V, KIMCHI-SARFATY C, SAUNA Z E, et al. P-glycoprotein: From genomics to mechanism. Oncogene[J].2003,22: 7468-7485. 
[6]  GOTTESMAN M M, FOJO T, BATES S E. Multidrug resistance in cancer: Role of ATP-dependent transporters[J]. Nature Reviews. Cancer, 2002(2): 48-58. 
[7]  MISRA R, ACHARYA S, SAHOO S K. Cancer nanotechnology: Application of nanotechnology in cancer therapy[J]. Drug Discovery Today, 2010,15: 842-850. 
[8]  SHAPIRA A, LIVNEY Y D, BROXTERMAN H J, et al. Nanomedicine for targeted cancer therapy: Towards the overcoming of drug resistance[J]. Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy, 2011,14: 150-163. 
[9]  DUNCAN R. Polymer conjugates as anticancer nanomedicines[J]. Nature Reviews Cancer. 2006(6): 688-701. 
[10]  CHITHRANI B D, GHAZANI A A, CHAN W C. Determining the size and shape dependence of gold nanoparticle uptake into mammalian cells[J]. Nano Letters, 2006(6): 662-668. 
[11]  CHITHRANI B D, CHAN W C. Elucidating the mechanism of cellular uptake and removal of protein-coated gold nanoparticles of different sizes and shapes[J]. Nano Letters, 2007(7): 1542-1550. 
[12]  WONG H L, BENDAYAN R, RAUTH A M, et al. A mechanistic study of enhanced doxorubicin uptake and retention in multidrug resistant breast cancer cells using a polymer-lipid hybrid nanoparticle system[J]. The Journal of Pharmacology and Experimental Therapeutics, 2006,317: 1372-1381. 
[13]  BONOMI P. Paclitaxel poliglumex (PPX, CT-2103): macromolecular medicine for advanced non-small-cell lung cancer[J]. Expert Review of Anticancer Therapy, 2007(7): 415-422. 
[14]  DUNCAN R, VICENT M J. Do HPMA copolymer conjugates have a future as clinically useful nanomedicines? A critical overview of current status and future opportunities[J]. Advanced Drug Delivery Reviews, 2010,62: 272-282. 
[15]  ETRYCH T, SIROVA M, STAROVOYTOVA L, et al. HPMA copolymer conjugates of paclitaxel and docetaxel with pH-controlled drug release[J]. Molecular Pharmaceutics, 2010(7): 1015-1026. 
[16]  MILLER K, ELDAR-BOOCK A, POLYAK D, et al. Antiangiogenic antitumor activity of HPMA copolymer-paclitaxel-alendronate conjugate on breast cancer bone metastasis mouse model[J]. Molecular Pharmaceutics, 2011(8): 1052-1062. 
[17]  VAN S, DAS S K, WANG X, et al. Synthesis, characterization, and biological evaluation of poly(L-gamma-glutamyl-glutamine)- paclitaxel nanoconjugate[J]. International Journal of Nanomedicine, 2010(5): 825-837. 
[18]  WANG X, ZHAO G, VAN S, et al. Pharmacokinetics and tissue distribution of PGG-paclitaxel, a novel macromolecular formulation of paclitaxel, in nu/nu mice bearing NCI-460 lung cancer xenografts[J]. Cancer Chemotherapy and Pharmacology,2010,65: 515-526. 
[19]  YANG D, VAN S, JIANG X, et al. Novel free paclitaxel-loaded poly(L-gamma-glutamylglutamine)-paclitaxel nanoparticles[J]. International Journal of Nanomedicine, 2011(6): 85-91. 
[20]  YANG D, VAN S, SHU Y, et al. Synthesis, characterization, and in vivo efficacy evaluation of PGG-docetaxel conjugate for potential cancer chemotherapy[J]. International Journal of Nanomedicine, 2012(7): 581-589. 
[21]  PAJIC M, IYER J K, KERSBERGEN A, et al. Moderate increase in Mdr1a/1b expression causes in vivo resistance to doxorubicin in a mouse model for hereditary breast cancer[J]. Cancer Research, 2009,69: 6396-6404. 
[22]  DUNCAN R. Polymer therapeutics as nanomedicines: New perspectives [J]. Current Opinion in Biotechnology, 2011,22: 492-501. 
[23]  WAN C P, LETCHFORD K, JACKSON J K, et al. The combined use of paclitaxel-loaded nanoparticles with a low-molecular-weight copolymer inhibitor of P-glycoprotein to overcome drug resistance[J]. Int J Nanomedicine, 2013(8): 379-391. 
[24]  CASCORBI I. P-glycoprotein: tissue distribution, substrates, and functional consequences of genetic variations[J].Handb Exp Pharmacol, 2011,201: 261-283.

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