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盘基网柄菌粘附分子gp150的定位及其与PKA活性关系的研究

, PP. 80-87

Keywords: 荧光定位,gp150,蛋白激酶A,HPLC

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Abstract:

细胞粘附分子gp150在盘基网柄菌细胞发育后期起着重要作用.用gp150抗体定位gp150在细胞发育重要阶段的分布,显示在细胞发育的不同时段,gp150的定位有着明显的区别.在聚集前的细胞流时期,gp150还均匀分布在细胞质内,到了细胞丘时期,gp150就移至多细胞聚集体的边缘部位.到了蛞蝓体时期,gp150在前柄细胞的表达量明显大于前孢子细胞,提示了gp150对柄细胞的作用.而当细胞发育至子实体阶段,gp150大量分布在成熟孢子的孢壁上,这是目前还没有报道的.高效液相色谱法检测野生型KAx-3细胞和gp150过表达细胞KAx-3:act15/lagC的PKA活性,显示在细胞发育的早期(10h之前),PKA在野生型细胞中的活性比KAx-3:act15/lagC细胞低.但是在细胞发育的后期,也就是野生型细胞中gp150开始快速积累之后,PKA在野生型细胞中的活性比KAx-3:act15/lagC细胞高.这些结果说明,gp150和PKA之间可能存在某种负反馈环的关系共同调节盘基网柄菌的生长发育.

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