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西太平洋“暖池”深海沉积物中异化型亚硫酸盐还原酶基因多样性分析

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Keywords: 西太平洋“暖池”,深海沉积物,异化型亚硫酸盐还原酶

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Abstract:

提取西太平洋"暖池"区深海沉积物样品总DNA,利用异化型亚硫酸盐还原酶(DSR)和甲基辅酶M还原酶(MCR)基因的特异引物,采用PCR-RFLP方法对沉积物样品中这两类功能基因多样性进行研究.该沉积物中的dsrAB基因分别来源于δ-变形菌中的6个属,其中最多的是脱硫弧菌属和脱硫杆状菌属;mcrA基因均来源于产甲烷古菌,其中主要是甲烷微菌.这些基因在系统发育树上都处于相对独立的分支.此外,还有较多的dsrAB,mcrA基因来源于未知的新属或新种.这些结果表明该海区沉积物中由微生物参与的硫和甲烷循环比较活跃,而且其中可能存在多种新的代谢途径.

References

[1]  RAVENSCHLAG K,SAHM K,KNOBLAUCH C,et al.Community structure,cellular rRNA content,and activity of sulfate-reducingbacteria in marine arctic sediments[J].Applied and Environmental Microbiology,2000,66:3592-3602.
[2]  KLEIN M,FRIEDRICH M,ROGER A J,et al.Multiple lateral transfers of dissimilatory sulfite reductase genes between maior lineages of sulfate-reducing prokaryotes[J].Journal of Bacteriology,2001,183:6028-6035.
[3]  曾润颖,赵晶,张锐,等.西太平洋"暖池"区沉积物中的细菌类群及其与环境的关系[J].中国科学(D辑),2004,34(3):265-271.
[4]  LANDRY M R,KIRCHMAN D L.Microbial community structure and variability in the tropical Pacific[J].Deep-Sea Research PartⅡ:Topical Studies in Oceanography,2002,49:2669-2693.
[5]  WAGNER M,ROGER A J,FLAX J L,et al.Phylogeny of dissimilatory sulfite reductases supports an early origin of sulfate respiration[J].Journal of Bacteriology,1998,180:2975-2982.
[6]  GIOVANNONI S J,MULLINS T D,FIELD K G.Microbial diversity in oceanic systems:rRNA approaches to the study of uncuhurable microbes[M].Molecular ecology of aquatic microbes.Berlin:Springer-Verlag Press,1995:217-248.
[7]  KJELDSEN K U,LOY A,JAKOBSEN T F,et al.Diversity of sulfate-reducing bacteria from an extreme hypersaline sediment,Great Salt Lake (Utah)[J].FEMS Microbiology Ecology,2007,80:287-298.
[8]  MULLER J A,GALUSHKO A S,KAPPLER A,et al.Initiation of anaerobic degradation of p-Cresol by formation of 4-hydroxybenzyl-succinate in Desulfobacterium cetonicum[J].Journal Bacteriology,2001,183:752-757.
[9]  JANSEN K,THAUER R K,WIDDEL F,et al.Carbon assimilation pathways in sulfate reducing bacteria.Formate,carbon dioxide,carbon monoxide,and acetate assimilation by Desulfovibrio baarsii[J].Archives of Microbiology,1984,138:257-262.
[10]  LUEDERS T,CHIN K J,CONRAD R,et al.Molecular analyses of methyl-eoenzyme M reductase alpha-suhunit (merA) genes in rice field soil and enrichment cultures reveal the methanogenic phenotype of a novel archaeal lineage[J].Environmental Microbiology,2001,3:194-204.
[11]  SIMANKOVA M V,KOTSYURBENKO O R,LUEDERS T,et al.Isolation and characterization of new strains of methanogens from cold terrestrial habitats[J].Systematic and Applied Microbiology,2003,26:3t2-318.
[12]  GRUIC-SOVULJ I,JARIC J,DULIC M,et al.Shuffling of discrete tRNASer regions reveals differently utilized identity elements in yeast and methanogenic arehaea[J].Journal of Mololecular Biology,2006,361:128-139.
[13]  DHILLON A,TESKE A,DILLON J,et al.Molecular characterization of sulfate-reducing bacteria in the Guaymas Basin[J].Applied and Environmental Microbiology,2003,69:2765-2772.
[14]  ORPHAN V J,HINRICHS K U,USSLER W,et al.Comparative analysis of methane-oxidizing Archaea and sulfate-reducing bacteria in anoxic marine sediments[J].Applied and Environmental Microbiology,2001,67:1922-1934.
[15]  陈骏,姚素平,季峻峰,等.微生物地球化学及其研究进展[J].地质论评,2004,50:620一632.
[16]  DHILLON A,TESKE A,DILLON J,et al.Molecular characterization of sulfate-reducing bacteria in the Guaymas BasinrJ\'].Applied and Environmental Microbiology,2003,69:2765-2772.
[17]  HALES B A,EDWARDS C,RITCHIE D A,et al.Isolation and identification of methanogen-specific DNA from blanket bog peat by PCR amplification and sequence analysis[J].Applied And Environmental Microbiology,1996,62:668-675.
[18]  MULLER J A,GALUSHKO A S,KAPPLER A,et al.Anaerobic degradation of m-cresol by Desulfobacterium cetonicum is initiated by formation of 3-hydroxybenzylsuccinate[J].Archives of Microbiology,1999,172:287-294.
[19]  FRIEDRICH M W.Phylogenetic analysis reveals multiple lateral transfers of adenosine-5"-phosphosulfate reductase genes among sulfatereducing microorganisms[J,].Journal of Bacteriology,2002,184:278-289.
[20]  IMACHI H,SAKAI S,SEKIGUCHI Y,et al.Methanolinea tarda gen.nov.,sp.nov.,a methane-producing archaeon isolated from a methanogenic digester sludge[J].International Journal Systematic and Evolutionary Microbiology,2008,58:294-301.
[21]  SINGH N,KENDALL M M,LIU Y,et al.Isolation and characterization of methylotrophic methanogens from anoxic marine sediments in Skan Bay,Alaska:description of Methanocoecoides alaskense sp.nov.,and emended description of Methanosarcina baltica[\'J].International Journal of Systematic and Evolutionary Microbiology,2005,55:2531-2538.
[22]  SZABOZ,STAHL A O,ALBERS S V,et al.Identification of diverse archaeal proteins with class Ill signal peptides cleaved by distinct arehaeal prepilin peptidases[J].Journal Bacteriology,2007,189:772-778.
[23]  D\'HONDT S,RUTHERFORD S,SPIVACK A J.Metabolic activity of subsurface life in deep-sea sediments[J].Science,2002,295:2067-2070.

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