全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

大菱鲆2种类胰岛素样生长因子结合蛋白基因克隆及在成鱼和早期发育期中的表达

, PP. 139-146

Keywords: 大菱鲆,类胰岛素样生长因子结合蛋白,基因克隆,mRNA表达

Full-Text   Cite this paper   Add to My Lib

Abstract:

类胰岛素样生长因子结合蛋白(IGFBP)在调节IGF的生物学功能上起着重要的作用,利用简并引物扩增及RACE克隆技术,首次克隆得到大菱鲆IGFBP-1,-2基因cDNA全长序列。两个基因氨基酸序列都分为保守的N端和C端以及高度特异的L区域3个部分,其中N端和C端共包含18个半胱氨酸残基,N端存在一段保守的(GCGCCXXC)结构,C端存在一段保守的(CWCV)结构,这些结构在与IGF相互作用以及调节与IGF结合的亲和性和稳定性起重要作用。组织表达分析表明,igfbp-1主要在肝脏及脾中大量表达,在其他组织中表达量较少,igfbp-2除在肌肉中没有检测到表达外,在其他组织中均有表达,其中肝脏脑、心、肾、肠中表达量较高。广泛的组织表达模式表明IGFBP是通过自分泌和旁分泌来调节IGF的功能。对于胚胎发育期表达分析表明,igfbp-1在整个发育期均检测到表达,igfbp-2在胚体期开始有表达,结果表明igfbp-1和igfbp-2在胚胎发育过程中的细胞生长以及组织器官分化都起着重要的生理学作用。

References

[1]  WERNER H, LEROITH D. The role of the insulin-like growth factor system in human cancer[J]. Adv Cancer Res, 1996, 68: 183-223.
[2]  JONES J I, CLEMMONS D R. Insulin-like growth factors and their binding proteins: biological actions[J]. Endocr Rev, 1995, 16: 3-34.
[3]  DUAN C, CLEMMONS D R. Differential expression and biological effects of insulin-like growth factor-binding protein-4 and-5 in vascular smooth muscle cells[J]. Biol Chem 1998 273: 16836-16842.
[4]  DUAN C, XU Q. Roles of insulin-like growth factor (IGF) binding proteins in regulating IGF actions[J] Gen Comp Endocrinol, 2005, 142: 44-52.
[5]  SCHNEIDER M R, WOLF E, HOEFLICH A, et al. IGF-binding protein-5: flexible player in the IGF system and affector on its own[J]. Endocrinol, 2002, 172: 423-440.
[6]  FIRTH S M, BAXTER R C. Cellular action of the insulin-like growth factor binding proteins[J]. Endocr Rev, 2002, 23: 824-854.
[7]  KAMANGAR B B, GABILLAR J C, BOBE J. Insulin-like growth factor-binding protein (IGFBP)-1,-2,-3,-4,-5, and-6, and IGFBP-related protein 1 during rainbow trout postvitellogenesis and oocyte maturation: molecular characterization, expression profiles, and hormonal regulation[J]. Endocrinol, 2006,147: 2399-2410.
[8]  PARK R, SHEPHERD B S, NISHIOKA R S. et al. Effects of homologous pituitary hormone treatment on serum insulin-like growth-factor-binding proteins (IGBPs) in hypophysectomized tilapia, Oreochromis mossambicus, with special reference to a novel 20-kDa IGFBP[J]. Gen Comp Endocrinol, 2000, 117: 404-412.
[9]  MAURES T J, DUAN C. Structure, developmental expression, and physiological regulation of zebrafish IGF binding protein-1[J]. Endocrinol, 2000, 143: 2722-2731.
[10]  SHIMIZU M, DICKEY J T, FUKADA H, et al. Salmon serum 22 kDa insulin-like growth factor-binding protein (IGFBP) is IGFBP-1[J]. Endocrinol, 2005, 184: 267-276.
[11]  FUNKENSTEIN B, TSAI W, MAURES T, et al. Ontogeny, tissue distribution, and hormonal regulation of insulin-like growth factor binding protein-2 (IGFBP-2) in a marine fish, Sparus aurata[J]. Gen Comp Endocrinol, 2002,128: 112-122.
[12]  CHENG R, CHANG K M, WU J L. Different temporal expressions of tilapia (Oreochromis mossambicus) insulin-like growth factor-I and IGF binding protein-3 after growth hormone induction. Mar[J]. Biotechnol, 2002,4: 218-225.
[13]  CLEMMONS D R. Insulin-like growth factor binding proteins and their role in controlling IGF actions[J]. Cytokine Growth Factor Rev, 1997,8 (1): 45-62.
[14]  雷霁霖,刘新富.大菱鲆Scophthalmus maximus L.引进养殖的初步研究[J]. 现代渔业信息, 1995,10 (11): 1-3.
[15]  陈彩芳,温海深,何峰,等.程序化设计的简并引物克隆半滑舌鳎CYP17基因[J].中国海洋大学学报,2009,39 (6): 1213-1218.
[16]  HOBBA G D, LOTHGREN A, HOLMBERG E, et al. Alanine screening mutagenesis establishes tyrosine 60 of bovine insulin-like growth factor binding protein-2 as a determinant of insulin-like growth factor binding[J]. Biol Chem, 1998, 273: 19691-19698.
[17]  JONES J I, GOCKERMAN A, BUSBY W H, et al. Insulin-like growth factor binding protein-1 stimulates cell migration and binds to the α5β1 integrins by means of its ARG-GLY-ASP sequence[J]. Proc Natl Acad Sci, 1993, 90: 10553-10557.
[18]  ARAI T, PARKER A, BUSBY JR W, et al. Heparin, heparan sulfate, and dermatan sulfate regulate formation of the insulin-like growth factor-I and insulin-like growth factor-binding protein complexes[J]. Biol Chem, 1994, 269: 20388-20393.
[19]  DUAN C, DING J, LI Q, et al. Insulin-like growth factor binding protein 2 is a growth inhibitory protein conserved in zebrafish[J]. Proc Natl Acad Sci, 1999, 96: 15274-15279.
[20]  CHEN W, LI W, LIN H. Common carp (Cyprinus carpio) insulin-like growth factor binding protein-2 (IGFBP-2): molecular cloning, expression profiles, and hormonal regulation in hepatocytes[J]. Gen Comp Endocrinol, 2009, 161: 390-399.
[21]  CHEN W, WANG Y, LIN H. Insulin-like growth factor binding protein-2 (IGFBP-2) in orange-spotted grouper, Epinephelus coioides: Molecular characterization, expression profiles and regulation by 17β-estradiol in ovary[J]. Comp Biochem
[22]  STEWART C E, ROTWEIN P. Growth, differentiation, and survival: multiple physiological functions for insulin-like growth factors [J]. Physiol Rev, 1996, 76: 1005-1026.
[23]  DUAN C, Specifying the cellular responses to IGF signals: roles of IGF binding proteins[J]. Endocrinol, 2002, 175: 41-54.
[24]  PARIKH S, KEIL M, ELBERG J. et al. Comparison of insulin sensitivity, clearance, and secretion estimates using euglycemic and hyperglycemic clamps in children[J]. J Clin Endocrinol Metab,2002,87(6):2978.
[25]  白雪.胰岛素样生长因子结合蛋白-2(IGFBP-2)的研究进展[J].国外医学神经病学神经外科学分册,2005,32(3):238-240.
[26]  万兵,闫杰,季明春,等.一种优化的PCR方法——降落PCR扩增目的基因[J]. 江苏临床医学杂志,2002,6 (2): 126-129.
[27]  孙业盈,张全启,齐洁,等.半滑舌鳎DMRT1基因的克隆与表达分析[J].武汉大学学报:理学版,2008,5倴栠礨猲椩漺氠?′㈱??????????????????ES E F, TURNER D, HODGE S J, et al. Localization of an insulin-like growth factor (IGF)binding site of bovine IGF binding protein-2 using disulfide mapping and deletion mutation analysis of the C-terminal domain[J]. Biol Chem, 1998,273: 4647-4652.
[28]  HWA V, OH Y, ROSENFELD R G. The insulin-like growth factor-binding protein (IGFBP) superfamily[J]. Endocr Rev, 1999, 20 (6): 761-787.
[29]  PEDROSO F L, FUKADA H, MASUMOTO T. Molecular characterization, tissue distribution patterns and nutritional regulation of IGFBP-1,-2,-3 and-5 in yellowtail, Seriola quinqueradiata[J]. Gen Comp Endocrinol, 2009, 161: 344-353.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133