全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

凡纳滨对虾大量EST的生物信息学分析

DOI: 10.3969/j.issn.0253-4193.2013.05.014, PP. 128-136

Keywords: 凡纳滨对虾,EST,基因组织特异性表达

Full-Text   Cite this paper   Add to My Lib

Abstract:

凡纳滨对虾是我国乃至世界最重要的对虾养殖种。EST(expressedsequencetags)测序是对没有基因组背景的生物进行功能基因分析最为有效的方法之一。为了研究凡纳滨对虾重要功能基因、以及为筛选分子标记奠定基础,本文集成了一套快速高效的EST信息深度挖掘的方法,并对公共数据库中161241条凡纳滨对虾EST序列进行分析,获得了20410条unigenes,包括14236条contigs和6174条singlets。通过与NR数据库比对发现有注释的基因7984条,并对其余12426条未知基因中的4702条进行了功能域注释。对有NR注释的基因的GO分类分析获得其中2715条基因的生物学过程、细胞定位和分子功能分类信息。此外,通过与模式生物通路图进行比对和定位,将3738条基因定位到270个KEGG通路图中,发现凡纳滨对虾的9条unigenes与果蝇丝裂原活化蛋白激酶(MAPK)通路的5个关键基因对应,提示凡纳滨对虾很可能存在类似的重要信号转导通路。另外,通过对6种组织,包括肝胰腺、淋巴器官、血细胞、鳃、眼柄和神经的EST进行比较分析,筛选获得了组织特异性转录本共7000余条,并对这些特异性转录本进行了功能分类。本研究不仅开发了公共基因资源发掘的方法,也为对虾功能基因的研究利用提供了重要参考数据。

References

[1]  Gross P S, Bartlett T C, Browdy C L, et al. Immune gene discovery by expressed sequence tag analysis of hemocytes and hepatopancreas in the Pacific White Shrimp, Litopenaeus vannamei, and the Atlantic White Shrimp, L. setiferus[J]. Developmental and Comparative Immunology, 2001,25(7): 565-577.
[2]  Xiang J H. Over 10,000 expressed sequence tags from Fenneropenaeus chinensis[R]//International Aquaculture Conference and Exposition, Beijing, 2002:101-102.
[3]  Cesar J R, Zhao B, Yang J. Analysis of expressed sequence tags from abdominal muscle cDNA library of the pacific white shrimp Litopenaeus vannamei[J]. Animal, 2008, 2(09):1377-1388.
[4]  Freitas P D, Pinheiro A P, et al. In silico analysis of polymorphic microsatellites in penaeid shrimp and construction of a free-access database[J]. Genetics and Molecular Biology, 2007, 30(4): 1194-1197.
[5]  Ciobanu D C, Bastiaansen J W, Magrin J, et al. A major SNP resource for dissection of phenotypic and genetic variation in Pacific white shrimp (Litopenaeus vannamei)[J]. Animal genetics, 2010, 41(1): 39-47.
[6]  Liang F, Holt I, Pertea G, et al. An optimized protocol for analysis of EST sequences[J]. Nucleic acids research, 2000,28(18): 3657-3665.
[7]  Ye J, Fang L, Zheng H, et al. WEGO: a web tool for plotting GO annotations[J]. Nucleic Acids Research, 2006,34: W293-297.doi:10.1093/nar/gk1031.
[8]  Kanehisa M, Goto S. KEGG: Kyoto Encyclopedia of Genes and Genomes[J]. Nucleic Acids Research,2000,28(1): 27-30.
[9]  Marchler-Bauer A, et al. CDD: a Conserved Domain Database for the functional annotation of proteins[J]. Nucleic Acids research, 2011,39(D): 225-229.
[10]  Zhang Y, Dong C. Regulatory mechanisms of mitogen-activated kinase signaling[J]. Cell Mol Life Sci, 2007,64(21): 2771-2789.
[11]  Noselli S, Agnès F. Roles of the JNK signaling pathway in Drosophila morphogenesis[J]. Current Opinion in Genetics and Development, 1999, 9(4): 466-472.
[12]  Dall W, Hill B J, Rothlisberg P C, et al. The biology of the penaeidae[J]. Advances in Marine Biology, 1990, 27: 1-489.
[13]  Adams M D, Kelley J M, et al. Complementary-DNA sequencing-expressed sequence tags and human genome project[J]. Science,1991, 252(5013): 1651-1656.
[14]  Lehnert S A, Wilson K J, Byrne K, et al. Tissue-Specific Expressed Sequence Tags from the Black Tiger Shrimp Penaeus monodon[J]. Marine Biotechnology, 1999,1: 465-476.
[15]  Rojtinnakorn J, Hirono I, Itami T, et al. Gene expression in haemocytes of kuruma prawn, Penaeus japonicus, in response to infection with WSSV by EST approach [J]. Fish & Shellfish Immunology, 2002,13(1): 69-83.
[16]  O\'Leary N A, Trent H F, Robalino J,et al. Analysis of multiple tissue-specific cDNA libraries from the Pacific whiteleg shrimp, Litopenaeus vannamei[J]. Integrative and Comparative Biology, 2006, 46(6): 931-939.
[17]  Perez F, Ortiz J, Zhinaula M, et al. Development of EST-SSR markers by data mining in three species of shrimp: Litopenaeus vannamei, Litopenaeus stylirostris, and Trachypenaeus birdy[J]. Mar Biotechnol (NY), 2005, 7(5): 554-569.
[18]  王艳红,胡超群,张吕平,等. 凡纳滨对虾EST微卫星标记初步筛选[J].水产学报, 2011, 35(7):969-976.
[19]  Gorbach D M, Hu Z L, Du Z Q, et al. SNP discovery in Litopenaeus vannamei with a new computational pipeline[J]. Animal Genetics, 2009, 40(1): 106-109.
[20]  Liu C Z, Wang X, Xiang J H, et al. EST-derived SNP discovery and selective pressure analysis in Pacific white shrimp (Litopenaeus vannamei)[J]. Chinese Journal of Oceanology and Limnology, accepted, 2012.
[21]  Pertea G, Huang X, Liang F, et al. TIGR Gene Indices clustering tools (TGICL): a software system for fast clustering of large EST datasets[J]. Bioinformatics, 2003,19(5): 651-652.
[22]  Ashburner M, Ball C A, Blake J A, et al. Gene ontology: tool for the unification of biology. The Gene Ontology Consortium[J]. Nature Genetics, 2000,25(1): 25-29.
[23]  Moriya Y, Itoh M, Okuda S, et al. KAAS: an automatic genome annotation and pathway reconstruction server[J]. Nucleic Acids Research, 2007,35(Web Server issue): W182-W185.
[24]  Biggs W H, et al. The Drosophila rolled locus encodes a MAP kinase required in the sevenless signal transduction pathway[J]. EMBO J, 1994,13(7): 1628-1635.
[25]  Adachi-Yamada T, et al. p38 mitogen-activated protein kinase can be involved in transforming growth factor β superfamily signal transduction in drosophila wing morphogenesis[J]. Mol Cell Biol, 1999,19(3): 2322-2329.
[26]  Peattie A M. Functional demands of dynamic biological adhesion: an integrative approach[J]. Journal of Comparative Physiology B, 2008,179(3): 231-239.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133