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中国沿海9种红藻hsp70基因序列及系统进化分析

DOI: 10.3969/j.issn.0253-4193.2013.04.022, PP. 188-200

Keywords: 红藻,hsp70,系统进化

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Abstract:

本研究自山东青岛、浙江象山和江苏南通采集共9种红藻样品,隶属于2纲、5目、6科、8属(据NCBI),克隆各红藻hsp70基因,并对所获序列进行分析。利用特异性引物P1/P3扩增,得到的目的条带约630bp,分析所推导的氨基酸序列发现:所获得片段均位于HSP70的ATPase结构域附近。9种红藻hsp70序列之间的遗传距离在0.078~0.319之间,序列相似度在73%~92%之间,其保守性略低于HSP70蛋白;基因对A或T结尾的密码子表现出很高的偏好性,CGC与TGG这两种密码子在这9种红藻HSP70氨基酸密码子中未出现。上述表明hsp70及HSP70密码子偏好性可应用于红藻分子系统学研究。基于多物种HSP70构建的进化树可见,Cyanidiumcaldarium与Cyanidioschyzonmerolaestrain10D两种原始红藻的起源早于其他红藻,紫菜次之,本研究中9种红藻系统发生符合NCBI的描述。在真菌、藻类和植物中,营养方式的差异可能是造成HSP70进化树分化的基本原因,而相同形态类型的物种中,环境适应是抗逆能力强、遗传结构稳定的物种生物分子进化的重要因素。

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