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白令海和楚科奇海表层沉积物中多环芳烃降解微生物多样性

DOI: 10.3969/j.issn.0253-4193.2014.04.002, PP. 52-61

Keywords: 白令海和楚科奇海,表层沉积物,多环芳烃,生物降解,生物多样性

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Abstract:

为了研究白令海(海盆及陆架)至楚科奇海陆架表层沉积物中多环芳烃(PAHs)降解菌的多样性,并获得新的PAHs降解菌资源。在GC-MS分析沉积物中PAHs种类和含量的基础上,以萘、菲和芘的混合物为唯一碳源和能源对表层沉积物样品进行富集,通过平板分离鉴定可培养菌株,并验证其降解能力;同时利用变性梯度凝胶电泳(DGGE)和Illumina高通量测序技术分析降解菌群结构。GC-MS测定结果表明,14个表层沉积物中PAHs总干质量介于32.99~276.97ng/g。富集菌群中共分离获得51株可培养细菌,平板纯培养、PCR-DGGE及Illumina测序结果均表明,菌群中优势的降解菌是γ-proteobacteria的Marinobacter,Pseudoalteromonas,Pseudomonas和Actinobacteria门的Dietzia菌。此外,Illumina测序结果还表明14个降解菌群在菌群结构组成上,可分为海盆区和陆架区两种类群;同时检测到一些低丰度的海洋专属PAHs降解菌,如Cycloclasticus,Alteromonas和Neptunomonas等。本文结果将加深对白令海及楚科奇海表层沉积物中PAHs降解菌资源与生物多样性的认识。

References

[1]  Baird C. Environmental Chemistry[M]. New York: W H. Freeman and Company,1995: 276—278.
[2]  Wang Z,Ma X,Na G,et al. Correlations between physicochemical properties of PAHs and their distribution in soil,moss and reindeer dung at Ny-Alesund of the Arctic[J]. Environ Pollut,2009,157(11):3132—3136.
[3]  Hung H,Blanchard P,Halsall C J,et al. Temporal and spatial variabilities of atmospheric polychlorinated biphenyls (PCBs),organochlorine (OC) pesticides and polycyclic aromatic hydrocarbons (PAHs) in the Canadian Arctic: results from a decade of monitoring[J]. The Science of the total environment,2005,342(1-3):119—144.
[4]  Whyte L G,Bourbonniere L,Greer C W. Biodegradation of petroleum hydrocarbons by psychrotrophic Pseudomonas strains possessing both alkane (alk) and naphthalene (nah) catabolic pathways[J]. Applied and environmental microbiology,1997,63(9):3719—3723.
[5]  Sorensen S R,Johnsen A R,Jensen A,et al. Presence of psychrotolerant phenanthrene-mineralizing bacterial populations in contaminated soils from the Greenland High Arctic[J]. FEMS microbiology letters,2010,305(2):148—154.
[6]  Eriksson M,Sodersten E,Yu Z,et al. Degradation of Polycyclic Aromatic Hydrocarbons at Low Temperature under Aerobic and Nitrate-Reducing Conditions in Enrichment Cultures from Northern Soils[J]. Applied and environmental microbiology,2003,69(1):275—284.
[7]  Cui Z,Lai Q,Dong C,et al. Biodiversity of polycyclic aromatic hydrocarbon-degrading bacteria from deep sea sediments of the Middle Atlantic Ridge[J]. Environmental Microbiology,2008,10(8):2138—2149.
[8]  Zhou H W,Li D F,Tam N F,et al. BIPES,a cost-effective high-throughput method for assessing microbial diversity[J]. ISME J,2011,5(4):741—749.
[9]  Jiang X T,Zhang H,Sheng H F,et al. Two-stage clustering (TSC): a pipeline for selecting operational taxonomic units for the high-throughput sequencing of PCR amplicons[J]. PloS one,2012,7(1):e30230.
[10]  Huse S M,Dethlefsen L,Huber J A,et al. Exploring microbial diversity and taxonomy using SSU rRNA hypervariable tag sequencing[J]. PLoS genetics,2008,4(11):e1000255.
[11]  Sogin M L,Morrison H G,Huber J A,et al. Microbial diversity in the deep sea and the underexplored "rare biosphere"[J]. Proceedings of the National Academy of Sciences of the United States of America,2006,103(32):12115—12120.
[12]  Witt G. Polycyclic aromatic hydrocarbons in water and sediment of the Baltic Sea[J]. Marine pollution bulletin,1995,31:237—248.
[13]  Wang B,Lai Q,Cui Z,et al. A pyrene-degrading consortium from deep-sea sediment of the West Pacific and its key member Cycloclasticus sp. P1[J]. Environ Microbiol,2008,10(8):1948—1963.
[14]  Bezalel L,Hadar Y,Fu P P,et al. Cerniglia CE: Initial Oxidation Products in the Metabolism of Pyrene,Anthracene,Fluorene,and Dibenzothiophene by the White Rot Fungus Pleurotus ostreatus[J]. Applied and environmental microbiology,1996,62(7):2554—2559.
[15]  Head I M,Jones D M,Roling W F M. Marine microorganisms make a meal of oil[J]. Nature Reviews Microbiology,2006,4(3):173—182.
[16]  Dong C,Chen L,Liao Y. Phylogenetic and degrading genes analysis of a PAHdegrading bacterium TVG9-Ⅶ from deep-sea hydrythermal environment[J]. Acta Microbiologica Sinica,2011,51(11):1548—1554.
[17]  Goldberg E D. Synthetic organohalides in the sea[J]. Proc R Soc Lond B Biol Sci,1975,189(1096):277—289.
[18]  Friedman C L,Selin N E. Long-range atmospheric transport of polycyclic aromatic hydrocarbons: a global 3-D model analysis including evaluation of Arctic sources[J]. Environmental science & technology,2012,46(17):9501—9510.
[19]  Ding X,Wang X M,Xie Z Q,et al. Atmospheric polycyclic aromatic hydrocarbons observed over the North Pacific Ocean and the Arctic area: Spatial distribution and source identification[J]. Atmospheric Environment,2007,41:2061—2072.
[20]  Halsall C,Barrie L,Jones K,et al. Modelling the behaviour of PAHs during atmospheric transport from the UK to the Arctic[J]. Atmospheric Environment,2001,35(2):255—267.
[21]  Wania F,Mackay D. Peer reviewed: tracking the distribution of persistent organic pollutants[J]. Environmental science & technology,1996,30(9):390A—396A.
[22]  Baumard P,Budzinski H,Garrigues P. Polycyclic aromatic hydrocarbons in sediments and mussels of the western Mediterranean Sea[J]. Environ Toxicol Chem,1998,17(5):765—776.
[23]  Pereira W E,Hostettler F D,Rapp J B. Distribution and fate of chlorinated insecticides,biomarkers and polycyclic aromatic hydrocarbons in sediments along a contamination gradient from a point-source in San Francisco Bay,California[J]. Mar Environ Res,1996,41∶299—314.
[24]  Cui Z,Shao Z. Predominant strains of polycyclic aromatic hydrocarbon-degrading consortia from deep sea of the Middle Atlantic Ridge[J]. Acta microbiologica Sinica,2009,49(7):902—909.
[25]  Hilyard E J,Jones-Meehan J M,Spargo B J,et al. Enrichment,isolation,and phylogenetic identification of polycyclic aromatic hydrocarbon-degrading bacteria from Elizabeth River sediments[J]. Appl Environ Microbiol,2008,74(4):1176—1182.
[26]  Giudice A L,Bruni V,Domenico M D,et al. Psychrophiles-cold-adapted hydrocarbon-degrading microorganisms[M]. Berlin:Springer, 2010: 1897—1921.
[27]  林学政,沈继红,杜宁,等. 北极海洋沉积物石油降解菌的筛选及系统发育分析[J]. 环境科学学报,2009,29(3):536—541.
[28]  Lin X,Yang B,Shen J,et al. Biodegradation of crude oil by an arctic psychrotrophic bacterium Pseudoalteromomas sp. P29[J]. Current microbiology,2009,59(3):341—345.
[29]  张月梅,祖国仁,那广水,等. 北极耐冷石油降解菌的筛选、鉴定及其碳源利用广谱性[J]. 海洋环境科学,2010,29(2):216—220.
[30]  Yakimov M M,Timmis K N,Golyshin P N. Obligate oil-degrading marine bacteria[J]. Curr Opin Biotechnol,2007,18(3):257—266.
[31]  Deppe U,Richnow H H,Michaelis W,et al. Degradation of crude oil by an arctic microbial consortium[M]. Extremophiles: life under extreme conditions,2005,9(6):461—470.
[32]  Gao W,Cui Z,Li Q,et al. Marinobacter nanhaiticus sp. nov.,polycyclic aromatic hydrocarbon-degrading bacterium isolated from the sediment of the South China Sea[J]. Antonie van Leeuwenhoek,2012.
[33]  Muyzer G,de Waal E C,Uitterlinden A G. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S RNA[J]. Appl Environ Microbiol,1993,59(3):695—700.

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