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冠瘤海鞘幼体附着和变态的离子控制

, PP. 137-144

Keywords: 冠瘤海鞘,幼体,附着,变态,离子控制,海鞘

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Abstract:

研究了人工海水中三种金属离子K+,Mg2+和Ca2+分别在不同浓度下对冠瘤海鞘(StyelacanopusSavigny)幼体附着和变态的影响,同时采用K+通道阻滞剂TEA检验其可兴奋细胞膜上的K+通道特性,对冠瘤海鞘幼体附着和变态的离子控制机制进行初步探讨,结果表明:K+的浓度为20~40mmol/dm3时诱导幼体附着和变态的效果最佳,低于45mmol/dm3和高于80mmol/dm3的K+浓度都表现出抑制效果;Mg2+浓度在0~32mmol/dm3时显著促进幼体附着和变态,达88mmol/dm3时起抑制作用;在人工海水中无论添加或减少Ca2+,均有效抑制幼体变态.TEA不能阻抑增加K+的诱导作用,这表明冠瘤海鞘幼体可兴奋细胞膜上的K+通道对外部TEA不敏感.另外,人工海水中缺乏K+或Ca2+或K+浓度达80mmol/dm3都会对冠瘤海鞘幼体产生毒害作用.

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