全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

赤魟软骨血管生成抑制因子的分离纯化

, PP. 113-118

Keywords: 赤魟,血管生成抑制因子,纯化,胶原酶,鸡胚绒毛尿囊膜

Full-Text   Cite this paper   Add to My Lib

Abstract:

确定了制备高纯度赤魟软骨血管生成抑制因子的纯化工艺,并通过胶原酶模型、CAM模型验证其生物学活性。正交实验确定了赤魟软骨组织抽提的最佳条件,抽提产率可达0.84%。丙酮分级沉淀对抽提产物进行粗分离,25~35%的丙酮分级产物的CAM血管生成抑制率达85%。优化离子交换层析、凝胶过滤、反相层析的条件,可进一步有效的获得高纯度的赤魟软骨血管生成抑制因子-I(DCAIF-I)。SDS-PAGE-考马斯亮蓝染色显示为一条带,分子量为62kDa。活性验证结果表明,DCAIF-I对胶原酶具有一定的抑制活性,抑制率为36.3%,对CAM血管生成的抑制活性具有一定的剂量依赖关系。

References

[1]  TAYLOR S,FOLKMAN J.Protamine is an inhibitor of angiogenesis[J].Nature,1982,297(27):307-312.
[2]  KUETTER K E,SOBLE L,CROXENTEAL R L,et al.Tumor cell collagenase and its inhibition by a cartilage-derived protease inhibitor[J].Science,1977,196(4290):653-654.
[3]  PEPPER M S,MONTESANO R M,VASSALLI JD.Chondrocytes inhibit endothelial sprout formation in vitro.,evidence for involvement of a transforming growth factor-beta[J].J Cel Physiol.1991,146(1):170-179.
[4]  贺国安,罗进贤,张添元,等.改进的鸡胚绒毛尿囊膜技术--无气室孵育法[J].中山大学学报(自然科学版),2003,42(2):126-128.
[5]  CAO Y.Tumor angiogenesia and therapy[J].Biomed Pharmacother,2005,59 (2):340-343.
[6]  BOEHM T,FOLKMAN J,BROWER T,et al.Antiangiogenic therapy of experimental cancer does not induce acquired drug resistance[J].Nature,1997,390; 404-407.
[7]  KERBEL.A cancer therapy resistant to resistance[J].Nature,1997,390 (6658):335-336.
[8]  FOLKMAN J.Tumor angiogenesis:Therapeutic implication[J].N Engl J Med,1971,285(21):1182-1186.
[9]  TAKIKAWA M,AHIRAI E,Enomotoetal M.Cartilage derived anti-tumor factor (CATF) inhibits the proliferation of endothelial cells in culture[J].Cel Bio Int Rep,1985,9(7):619-625.
[10]  LANGER R,BREM H,FALTERMANETAL K,et al.Isolation of a cartilage factor that inhibits tumor neo-vascularization[J].Science,1976,193:70-72.
[11]  郭斌,唐金中,贾玉海,等.魟鱼骨素对鸡胚绒毛尿囊膜血管形成的影响[J].辽宁中医杂志,2002,29(11),688-689.
[12]  郭斌,唐金中.贾玉海,等.魟鱼骨素抗肿瘤作用的初步研究[J].辽宁中医杂志.2002,29(6):368-369.
[13]  SAMBROOK J,FIRTSCH E F,MANIAIATRS T.Molecular Cloning:A Laboratory Manual,(2nd ed.)[M].New York:Cold Spring Harbor Laboratory Press,1989:47-58.
[14]  BARINAGA M.Peptide-guided cancer drugs show promise in mice[J].Science,1998,279:323-324.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133