GIULIETTI A,OVERBERGH L,VALCKX D,An overview of real-time quantitative PCR:applications to quantify cytokine gene expression,Methods:A Companion to Methods in Enzymology,2001(4).
Vandesompele J;de Preter K;Pattyn F;Poppe B van Roy N de Paepe A Speleman F,Accurate normalization of real-Ume quantitative RT-PCR data by geometric averaging of multiple internal control genes,Genome Biology？ ,2002, 3.
[4]
ANDERSEN C L,JENSEN J L,(Φ)RNTOFT T F. Normalization of real-time quantitative reverse transcription-PCR data:a model-based variance estimation approach to identify genes suited for normalization,applied to bladder and colon cancer data sets[J].Cancer Research,2004,(15):5245-5250.
[5]
Pfaffl MW ；Tichopad A ；Prgomet C ；Neuvians TP,Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper - Excel-based tool using pair-wise correlations,Biotechnology Letters？,2004, 26(6).
[6]
Kim BR ；Nam HY ；Kim SU ；Kim SI ；Chang YJ,Normalization of reverse transcription quantitative-PCR with housekeeping genes in rice,Biotechnology Letters？,2003, 25(21).
[7]
DHEDA K,HUGGETT J F,BUSTIN S A,Validation of housekeeping genes for normalizing RNA expression in real-time PCR,Biotechniques,2004(1).
Bustin,SA ；Benes,V ；Garson,JA ；Hellemans,J ；Huggett,J ；Kubista,M ；Mueller,R ；Nolan,T ；Pfaffl,MW ；Shipley,GL ；Vandesompele,J ；Wittwer,CT,The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments.,Clinical Chemistry？,2009, 55(4).
[14]
Lefever S ；Hellemans J ；Pattyn F ；Przybylski DR ；Taylor C ；Geurts R ；Untergasser A ；Vandesompele J,RDML: structured language and reporting guidelines for real-time quantitative PCR data,Nucleic Acids Research？,2009, 37(7).
[15]
GUTIERREZ L,MAURIAT M,GU(E)NIN S,The lack of a systemic validation of reference genes:a serious pitfall undervalued in reverse transcription-polymerase chain reaction (RT-PCR) analysis in plants,Plant Biotechnology Journal,2008(6).
[16]
Wood SH ；Clements DN ；McEwan NA ；Nuttall T ；Carter SD,Reference genes for canine skin when using quantitative real-time PCR,Veterinary Immunology and Immunopathology？,2008, 126(3/4).
