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一种测定龙眼果柄离层纤维素酶活性的方法

DOI: 10.7671/j.issn.1001-411X.2012.02.012, PP. 175-177

Keywords: 纤维素酶,离层,凝胶扩散-组织印迹法

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Abstract:

介绍一种测定离层纤维素酶活性的新方法—凝胶扩散-组织印迹法.给出了该测定方法的基本原理、流程、步骤及计算方法,并以此方法测定了龙眼果实脱落过程中果柄离层纤维素酶活性的变化.结果表明该方法需要材料少,灵敏度高,纤维素酶活性最低可检出2×10-6U.

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