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高效液相色谱法测定牛奶及牛可食性组织中咪唑苯脲

DOI: 10.7671/j.issn.1001-411X.2012.02.025, PP. 239-243

Keywords: 高效液相色谱,咪唑苯脲,残留,牛奶,牛可食性组织

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Abstract:

建立了高效液相色谱法(HPLC)测定牛奶及牛可食性组织中咪唑苯脲(IMD)残留.牛奶或已匀浆组织先用β-葡萄糖苷酶水解，组织用盐酸超声提取，再用提取液萃取，牛奶直接用提取液提取.用乙腈-戊烷磺酸钠水溶液作为流动相，经高效液相色谱C18柱分离后用紫外检测器检测.咪唑苯脲质量浓度在0.01～10.00μg/mL的范围内呈现良好的线性关系，相关系数大于0.999；组织加标样品检出限为0.01μg/g,定量限为0.02μg/g.在定量限、最高残留限量、2倍最高残留限量添加水平下的平均回收率为80.02%～114.72%，批内相对标准偏差为0.83%～10.92%，批间相对标准偏差为7.45%～12.69%.表明该检测方法简单、灵敏、可靠，适用于牛奶及牛组织中咪唑苯脲残留的分析.

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