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侵染广东黄秋葵的木尔坦棉花曲叶病毒及伴随卫星DNA的分子特征

DOI: 10.7671/j.issn.1001-411X.2012.01.007, PP. 33-39

Keywords: 黄秋葵黄脉曲叶病,木尔坦棉花曲叶病毒,DNA-A,卫星DNAβ

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Abstract:

从广东省表现黄脉曲叶的黄秋葵病株上分离到病毒分离物Okra06,PCR检测结果显示,该病毒属双生病毒科Geminiviridae菜豆金色花叶病毒属Begomovirus.基因克隆及序列分析结果表明,其基因组仅含A组分(DNA-A),全长为2737nt,推导编码6个开放阅读框(Openreadingframe,ORF).该组分与木尔坦棉花曲叶病毒(CottonleafcurlMultanvirus,CLCuMV)分离物G6的核苷酸序列相似性最高,为99.7%;二者编码的6个ORF相似性分别为100%、100%、99.6%、99.8%、100%和99.7%。该病毒还伴随有卫星分子β(DNAβ),全长为1346nt,推导其互补链上编码1个ORF(C1)。该分子与伴随CLCuMV分离物G6DNAβ的核苷酸序列相似性也最高(99.5%).DNAβ系统进化分析显示,Okra06DNAβ与CLCuMV-[G6]DNAβ、CLCuMV-[Gx08]DNAβ亲缘关系最近,三者形成一个独立分支;进一步与其他CLCuMVDNAβ和CLCuVDNAβ聚类在一起.这些结果证明,侵染广东黄秋葵的病毒分离物Okra06属于CLCuMV,且该分离物与引起广东朱槿曲叶病的CLCuMV-G6应同属木尔坦棉花曲叶病毒朱槿株系。

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