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动物血浆和组织中磺胺喹恶啉的HPLC测定-不同样本间药物含量的回归分析

DOI: 10.3321/j.issn:1000-7091.1989.02.018, PP. 102-107

Keywords: 磺胺喹恶啉,高效液相色谱,回归分析,间接预测

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Abstract:

采用高效液相色谱(HPLC)分析法测定动物血浆和组织中的磺胺喹恶啉,其准确度与专属性好,最低检出限为1×10-7g.色谱条件:以十八硅烷磺酸钠(ZorbaxODS反相柱)为固定相,紫外检测波长254nm,流动相为甲醇:水(50:50),流速为1ml/分,磺胺二甲基嘧啶作内标物.通过血浆与组织SQ含量间的回归分析,还建立多种回归方程,为组织中药物含量的间接预测提供计算依据.

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