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冬花椰菜的小孢子胚胎发生研究

DOI: 10.3321/j.issn:1000-7091.1990.03.010, PP. 48-51

Keywords: 冬花椰菜,花药培养,小孢子,胚胎发生

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Abstract:

应用花药培养技术在四个冬花椰菜(BrassicaoleraceaL.ssp.botrytis)基因型中获得小孢子胚胎.培养基中加入硝酸银能明显促进胚胎发生.在含125mg/1硝酸银的培养基上胚产量最高.培养基中加入0.5g/1活性碳,能提高小孢子胚胎发生频率.另外在培养基中用琼脂糖代替琼脂,也能提高胚产量.

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