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小麦天然钙调素抗体制备及酶标免疫组化定位

DOI: 10.3321/j.issn:1000-7091.1991.03.014, PP. 76-80

Keywords: 小麦,钙调素,钙调素抗体,酶标免疫组化定位

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Abstract:

以小麦不修饰的天然钙调素(CaM)制备抗体.免疫学特性分析表明,小麦CaM抗体不能与牛脑CaM发生交叉反应,而可与多种植物CaM发生良好的交叉反应,即所得抗体对多种植物CaM是专一的.酶标免疫组化法在烟草愈伤组织细胞定位表明,细胞质CaM含量较高|在细胞核中,特别是核仁中亦含有CaM.

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