生物素标记寡核苷酸探针检测和鉴定单核细胞增多症李氏菌的研究
DOI: 10.3321/j.issn:1000-7091.1994.z1.015, PP. 71-74
Keywords: 生物素,DNA探针,斑点杂交,单核细胞增多症李氏菌,检测,鉴定
Abstract:
采用寡核苷酸生物素探针进行了斑点杂交试验。结果表明，12株单核细胞增多症李氏菌均与探针呈杂交阳性，其它种李氏菌和细菌无一与探针杂交。探针检测靶DNA的敏感性为10ng，过夜增菌培养，可提高敏感性10~3～10~4倍，测出样品中少至150个细菌，探针法检测市售猪肉和牛奶的阳性率高于分离培养法，具有推广应用价值。
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