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高频率生菜植株再生及转化体系的建立

DOI: 10.3321/j.issn:1000-7091.1996.01.020, PP. 109-113

Keywords: 生菜,再生,遗传转化,农杆菌

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Abstract:

通过对两种生菜及其不同外植体在培养基上不定芽发生能力的筛选,得到“大湖366”叶切片不定芽发生率高达87%的培养体系,并且丛生状不定芽可陆续发生。将叶切片与农杆菌共培养,对经km筛选后所得再生株进行X-Glu染色,兰色反应阳性率为50%。

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