全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

乳牛精子细胞核的两性分离试验

DOI: 10.3321/j.issn:1000-7091.1997.04.026, PP. 126-129

Keywords: 乳牛,X-精子,Y-精子,细胞核,分离

Full-Text   Cite this paper   Add to My Lib

Abstract:

由于雄性配子(精子)有性染色体的差异,哺乳动物X、Y精子在质量或密度上存在着微小的差别。为了突出这微小的质量差,实验先将两性精子共有的顶体和尾部都去掉,然后再将精子细胞核在氯化铯密度梯度条件下离心,成功地将乳牛精子细胞核分成明显的两个区带。

References

[1]  Garner DL,Gledhill BL,Pinke lD. Quantification of the X-and Y-chromosome- bearing spermatozoa of domestic an imals by flow cytometry. Biology of Repro duction,1983,28:312~321.
[2]  Bhattacha ry aBC. The rmal convection counter streaming sed imentation and for cedconv ection galvan ization method and apparatus fo r controlling the sex of mam malian offspring. United States Paten t,1979,4155831.
[3]  Rescission RJR. S ex selection via album in columns:20 years of results(comment). Hum an Repro duction, 1994,9(10):1787~1788.
[4]  HunterM J,Commer ford SL. Pressure homogenization of mamm aliantissues. Biochimicaet Biophy sicaActa,1961,47:580~586.
[5]  Hefse U C,Shasry PR,Raao SS. Use of dithiothreitol for im proved visibility of the Fbodyin human Ybea ring sperm atozoa. J Reprod Fert,1978,53:403~405.
[6]  John son LA,Flook J P,LookM V. Flow cytom etry of X and Y chromosome- bea ring sperm fo r DNA using an improved prepa ration method and staining with hoechst 33342. Gamete Research,1987,17:302~212.
[7]  Van Kooij RJ,Van Oost BA. Determi nation of sex ratio of spermatozoa with a deoxy ribonucleic acidp robe and quinacrine staining:a comparison. Fertility and Sterility,1992,58(2):384~386.
[8]  刘嗣枢. 电泳法分离牛 X 和 Y 精子的初步研究. 辽宁畜牧兽医, 1985(4):9~10.
[9]  宋平根,李素文. 流式细胞术的原理和应用. 北京:北京师范大学出版社,1992,167~168.
[10]  Br andr iff BF. Sexch romosome ratiosdeter mined by kary o typicanaly sisinalbum inisolated hum ansperm. Fertility and Sterility,1986,46(4):678~685.
[11]  Kirkpatr ick BW,Monson RL. Sen sitive sex deter mination assay app licable to bovin embryo s de rived from IVM and IVF. Journal of Reproduction and Fertility,1993,98:335~340.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133