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利用大白菜小孢子胚状体获得抗除草剂转基因植株

DOI: 10.3321/j.issn:1000-7091.1998.04.017, PP. 93-98

Keywords: 大白菜,小孢子胚胎,根癌农杆菌,bar基因,除草剂抗性,Southern杂交

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Abstract:

大白菜小孢子培养获得的子叶期胚状体,经粉碎的玻璃碴摩擦后,与农杆菌共培养,在加筛选剂Basta(含phosphinothricin5mg/L)的培养基上,再生出数株绿苗。自交留种后,对其后代苗期进行的Basta抗性鉴定显示,有4株抗此除草剂。Southern杂交结果显示,抗性植株的基因组中各有一个bar基因插入位点。对转化株的小孢子进行再培养,后代小孢子植株对Basta抗性的分离比显示,此转基因株为杂合体。

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