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绿菜花游离小孢子培养、胚胎发生和植株再生

DOI: 10.3321/j.issn:1000-7091.1998.03.020, PP. 102-106

Keywords: 绿菜花,游离小孢子培养,胚胎发生,植株再生

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Abstract:

取花蕾长3.0~5.5mm的花序,7%(W/V)饱和次氯酸钙溶液(加一滴吐温20)表面消毒15min,无菌水洗3次。然后轻压花蕾,过滤,收集滤液,离心后,将小孢子悬浮于经过滤灭菌的改良的1/2NLN培养基中。石蜡膜封口,置温度梯度培养箱作32.5℃、1d的高温热激诱导处理,后置25℃下继续培养。本试验对13份绿菜花供试材料的游离小孢子培养能力进行初步研究,其中8种基因型(占全部材料的61.5%)有胚胎发生,并获得5种基因型的再生株,共207株。基因型对绿菜花游离小孢子培养的影响很大,基因型“巴绿青花菜”出胚率最高,达37.08胚/蕾。同时,对培养基中加入活性炭悬浮液和更换培养基的作用进行了研究。

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