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苹果休眠茎尖的超低温保存研究

DOI: 10.3321/j.issn:1000-7091.1999.01.026, PP. 129-133

Keywords: 苹果,休眠茎尖,超低温

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Abstract:

苹果金冠品种的休眠茎尖经3种不同方法(玻璃化法、包埋-干燥法和两步降温法)超低温保存后,均获得成功,存活率达到了83%。休眠茎尖由于经受了冬季的深冷驯化和具有较低的含水量,因而成为超低温保存的良好试材。经过玻璃化液(PVS3:50%甘油+50%蔗糖)预处理80min(25℃条件下)后或经蔗糖溶液预培养并于无菌空气中干燥脱水至含水量30%左右后,休眠茎尖可直接投入液氮进行超低温保存。保存后的休眠茎尖经离体培养后可直接再生植株。试验进一步验证了试材生理状态的选择是超低温保存成功的一个关键因素,这不仅可以简化超低温保存的程序,而且可以提高此方法的实用性,为早日建立植物种质的超低温保存库奠定了基础。

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