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影响绿菜花游离小孢子培养的因素

DOI: 10.3321/j.issn:1000-7091.1999.01.014, PP. 68-72

Keywords: 绿菜花,小孢子培养,影响因素

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Abstract:

对绿菜花“Galaxy”、“bc-0”和“bc-4”3个基因型进行了渗透压、外源激素、热激处理温度和时间、花蕾长度或小孢子发育时期的影响因素研究。结果表明,激素的作用并不明显,但相比之下,单独加入6-BA比同时加入6-BA、NAA效果好;绿菜花游离小孢子培养的最适热激处理温度和时间为32.5℃,24h;绿菜花游离小孢子的最适培养花蕾长度因基因型而异,如基因型“bc-4”蕾长为4.1~5.0mm,此时大多数小孢子处在单核晚期、双核早期,出胚率高达37.08胚/蕾。

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