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水蛭素的全基因合成及克隆

DOI: 10.3321/j.issn:1000-7091.2000.04.012, PP. 58-61

Keywords: 水蛭素,基因,合成,克隆

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Abstract:

报道了水蛭素全基因的合成及克隆。根据已知水蛭素氨基酸序列及酵母菌体内蛋白质表达特性,设计了水蛭素的全基因序列,采用化学合成及酶学相结合的方法,合成了该基因片段,并逐段克隆至载体pBS上,得到全长水蛭素基因。序列测定表明,克隆后DNA顺序同原设计序列。

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