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利用RAPD标记鉴定大白菜杂交种纯度的研究

DOI: 10.3321/j.issn:1000-7091.2000.04.008, PP. 35-39

Keywords: 大白菜,RAPD标记,杂交种纯度

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Abstract:

应用随机扩增多态性DNA(RAPD)标记鉴定大白菜杂种商品种子的纯度。用50个随机引物检测了2个杂交种北京57号和北京106号及其亲本,引物OPE-20在北京57号杂交种中产生了有别于双亲的特殊标记,引物OPH-06及OPH-07在北京106杂交种中产生了有别于双亲的特殊标记,能清楚地区分杂交种及其双亲,并将这些引物应用在北京57号和北京106号杂交种样品的纯度检测中。这个结果显示了RAPD标记在大白菜杂交种商品种子纯度检测上的实际用途。

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